1.2 Precipitation and Agglutination Reactions part 2 Flashcards
(26 cards)
Detects unknown antibodies in patirent’s serum using known antigens bound to a particulate
Passive agglutination
What is the unknown in passive agglutination?
Antibodies
Detects unknown antigens in the patient’s serum using known antibodies bound to particulate carrier
Reverse passive agglutination
What is the unknown in reverse passive agglutination?
Antigen
generally used in the blood bank for RBC phenotyping and antibody screening
Anti-human Globulin test / Coomb’s test
The AHG attach to what part of another antibody?
Fc portion
What type of immunoglobulin is AHG?
anti-IgG
Detects in VIVO sensitization of RBC
Direct AHG test
This test is used to detect autoimmune RBC?
Direct AHG test
Detects in Vitro sensitization of RBC
Indirect AHG test
AHG test that is used in cross matching or antibody screening identification
Indirect AHG test
What is the specimen for indirect AHG test?
Patient serum
What is the specimen for direct AHG test?
Patient RBCs
Based on competition between particulate and soluble antigens for limited antibody-combining sites
Agglutination inhibition
This test has Two-step procedure?
Agglutination inhibition
The first step in agglutination inhibition is not visible?
T
What is the positive result of agglutination inhibition?
No agglutination
Uses the same principle except that RBCs are used as indicator particles
Used to detect antibodies to certain viruses such as rubella, influenza and RSV
Hemagglutination inhibition
What is the positive result for Hemagglutination inhibition?
No agglutination
What is the indicator particle used in hemagglutination inhibition?
RBCs
What is the indicator particle used in hemagglutination inhibition?
RBCs
Based on the principle that antigen-antibody complexes bind or fix the complement and are unable to react with sensitized indicator cells
Complement fixation
What immuoglobulin is applicable only in complement fixation?
IgM
What is the positive result for complement fixation?
No hemolysis
because complement attach to antibody (This prevents attachment to RBC to avoid hemolysis)
