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Bacte Lec [Finals] > 1.2.1 Lab Diagnosis for Haemophilus > Flashcards

1.2.1 Lab Diagnosis for Haemophilus Flashcards

1
Q

How the specimen for Haemophilus is being collected?

A

Swab premoistened with sterile phosphate buffered saline, Stuart’s or Amie’s Swab

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2
Q

Culture media for Haemophilus?

A

Chocolate Agar
Chocolate Agar with Bacitracin
Chocolate Agar with 1% IsoVitaleX
Nairobi Biplate Medium

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3
Q

Why is chocolate Agar is the media of Choice for Haemophilus?

A

X and V factor

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4
Q

What is the function of bacitracin with Chocolate agar?

A

Inhibits the growth of oral respiratory normal flora

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5
Q

Chocolate agar with 1%IsoVitaleX is used for what bacteria?

A

H. ducreyi and H. aegypticus

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6
Q

Nairobi Biplate medium is specific for what Haemophilus?

A

H. ducreyi

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7
Q

Composition of Nairobi Biplate medium

A

GC agar base with 2% bovine hemoglobin
5% fetal calf serum
MHA with 5% chocolatized horse blood
Vancomycin

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8
Q

Function of Vancomycin in nairobi Biplate medium?

A

Resistant for H. ducreyi so it can grow while it can inhibit other microorganisms (Especially Gram positive)

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9
Q

What is the Incubation for Haemophilus spp. (Except ducreyi)?

A

5-10% Co2 at 35 -37C

24-72 hrs

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10
Q

Incubation for H. ducreyi?

A

5-10% Co2 at 33 C with high humidity

7 days

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11
Q

Small, Gram-negative coccobacilli to long filaments

Encapsulated

A

Haemophilus spp

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12
Q

Gram negative coccobacilli that appear as the following genital lesions?

School of Fish
Railroad tracks
Fingerprints

A

H. ducreyi

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13
Q

Colonial morphology:

Non hemolytic
Translucent, tannish, moist, smooth, and convex
Mousy / Bleach like odor in CHOC agar

A

H. influenzae

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14
Q

In H. influenzae, what is the indication if there is mucoid?

A

Encapsulated strains

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15
Q

Colonial morphology:
Small, Flat, Smooth, Transparent to opaque
Clumpy, non-homogenous appearance in saline

(Difficult to remove in CHOC)

A

H. ducreyi

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16
Q

Haemophilus spp grow on MAC?

A

False

17
Q

Lab diagnosis for Haemophilus?

A

Satellitism test
X and V test
Porphyrin test

18
Q

Use for routine identification or differentiation of Haemophilus spp.

A

Satellitism test

19
Q

How to streak in Satellitism?

A

Staphylococcus streak

20
Q

Haemophilus is streaked with S. aureus, S. pneumoniae, and Neisseria (All are V factor producer)

A

Staphylococcus streak

21
Q

What is the positive indicator for Satellitism test?

A

Moist, Small “Dewdrop” colonies surrounding the S. aureus colonies

22
Q

What bacteria is negative in Satellitism test?

A

H. haemolyticus
H. ducreri

23
Q

What media is used in Satellitism test?

A

SBA

24
Q

Incubation for Satellitism test?

A

72 hrs at 35 -37C

25
Q

What are the Haemophilus that are positive in both X and V factor?

A

H. influenzae
H. aegypticus
H. haemolyticus

26
Q

What are the haemophilus that are positive in V factor?

A

H. parainfluenzae
H. paraphrophilus
H. parahaemolyticus

27
Q

What are the haemophilus that are positive in X factor?

A

H. ducreyi

28
Q

What are the haemophilus that are Negative in both X and V factor?

A

H. aphrophilus

29
Q

What is the Media use for X and V factor?

A

MHA

30
Q

What is the reagent for Porphyrin tesT?

A

Aminolevulinic acid

31
Q

NOTE: Aminolevulinic acid -> Porphyrin -> Hemin

A
32
Q

Phorphobilinogen is detected using?

A

Kovac’s reagent

33
Q

What contains in Kovac’s reagent?

A

P-dimethylaminobenzaldehyde

34
Q

Positive indicator for Porphyrin test?

A

FLuoresce reddish orange at UC light (360nm)

35
Q

How many hrs of incubation for prophyrin test?

A

4 hrs

36
Q

Positive in Porphyrin test indicates?

A

Bacteria can produce X factor (Hemin)

37
Q

What do you call the organisms that is positive in Porphyrin test?

A

Heme producing species

38
Q

What are the bacteria that are positive in Hemolysis?

A

H. haemolyticus
H. parahaemolyticus

39
Q

Organisms that are positive in ALA?

A

H. parainfluenza
H. parahaemolyticus
H. paraphrohaemolyticus
H. aphrophilus
A. segnis

HINT: Lahat sila negative sa X factor since kaya nila mag produce ng sarili nilang X factor

Bacte Lec [Finals] (59 decks)

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