2.4.2 Laboratory diagnosis

Question 1

What is the specimen of choice for Bordetella?

Answer 1

Nasopharyngeal aspirates or swabs

Question 2

What is the transport medium for the specimen of choice?

Answer 2

Calcium alginate and Dacron

Question 3

NOTE: Throat swab can also be use for specimen collection

Question 4

What are the transport medium used?

Answer 4

Amies with charcoal
1% Casein hydrolysate
Regan-Lowe transport medium

Question 5

NOTE: Regan Lowe transport medium contains 10% horse blood and 40mg/L Cephalexin

Question 6

What are the Agar media use?

Answer 6

Bordet-Gengou/Potato blood glycerol
Charcoal horse blood

Question 7

Incubation?

Answer 7

Moist chamber at 35C > 7days

Question 8

What is the microscopical morphology for Bordetella?

Answer 8

Tiny gram neg coccobacilli

Can also use Direct fluorescent antibody staining

Question 9

What media is used to identify hemolytic for Bordetella?

Answer 9

Bordet gengou agar

Question 10

What agar can give these characteristics?

Smooth, Glistening and silver, Resembing mercury droplets

Answer 10

Charcoal-horse blood and Regan Lowe media

Question 11

How many days for the growth of B. pertussis in Charcoal Horse blood agar?

Answer 11

3-5 days

Question 12

How many days for the growth of B. parapertussis in Charcoal Horse blood agar?

Answer 12

2-3 days

Question 13

Charcoal horse blood (3-5 days)
Catalase (+)
Oxidase (+)
Urease (-)
Nitrate (-)
Motility (-)

Answer 13

B. pertussis

Question 14

Charcoal horse blood (2-3 days)
Catalase (+)
Oxidase (-)
Urease (+)
Nitrate (-)
Motility (-)

Answer 14

B. parapertussis