3.4.EX Lab diag (Gram neg bacilli)

Question 1

Cellular morphology:
Coccobacilli / Pleomorphic

Colony morphology:
Non hemolytic
Large gray black colonies in BBE

Answer 1

Bacteroides fragilis

Question 2

Cellular morphology:
Tiny coccobacilli

Colony morphology:
Fluoresces brick red on blood agar
Black pigment in KVLB agar

Answer 2

Prevotella spp.

Question 3

Cellular morphology:
Spindle-shaped with pointed ends

Colony morphology:
Colony types: Bread crumblike / Ground glass
Green colonies when exposed to air
Fluoresces chartreuse

Answer 3

Fusobacterium nucleatum

Question 4

Vancomycin (Res)
Kanamycin (Res)
Colistin (Res)
Bile resistance (+)
Esculin hydrolysis (+)
Growth on KVLB (+)
Brick Red Fluorescence (-)
Chartreuse Fluorescence (-)
Iridescent sheen (-)

Answer 4

B. fragilis

Question 5

Vancomycin (Res)
Kanamycin (Res)
Colistin (Sus / V)
Bile resistance (-)
Esculin hydrolysis (-)
Growth on KVLB (+)
Brick Red Fluorescence (+)
Chartreuse Fluorescence (-)
Iridescent sheen (-)

Answer 5

Prevotella spp.

Question 6

Vancomycin (Res)
Kanamycin (Sus)
Colistin (Sus)
Bile resistance (-)
Esculin hydrolysis (-)
Growth on KVLB (-/+)
Brick Red Fluorescence (-)
Chartreuse Fluorescence (+)
Iridescent sheen (+)

Answer 6

Fusobacterium nucleatum

Question 7

Vancomycin (Sus)
Kanamycin (Res)
Colistin (Res)
Bile resistance (-)
Esculin hydrolysis (-)
Growth on KVLB (-)
Brick Red Fluorescence (+)
Chartreuse Fluorescence (-)
Iridescent sheen (-)

Answer 7

Porphyromonas spp.