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Bacte Lec [Finals] > 3.2.2 Clostridium perfringens > Flashcards

3.2.2 Clostridium perfringens Flashcards

1
Q

Virulence factor that is responsible for Food poisoning?

A

Beta and Alpha toxin

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2
Q

In Beta and Alpha toxin, what type that is mild and can cause diarrhea and cramps?

A

Type A

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3
Q

In Beta and Alpha toxin, what type that cause Enteritis necroticans?

A

Type C

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4
Q

What is the incubation period when develop the symptoms of abdominal pain, bloody diarrhea and vomiting in Enteritis necroticans?

A

5-6 hrs

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5
Q

Type C / Enteritis necroticans is caused by what specific toxin?

A

Beta toxin

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6
Q

What is the toxin responsible for necrosis of tissue and deeper penetration?

A

Alpha toxin

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7
Q

Alpha toxin = Necrosis tissue

Signs and symptoms:
Bullae - fluid filled blisters
Serous discharge
Discoloration
Tissue necrosis (Increased gas inside wound infection)

What is the disease?
What is the bacteria that cause this?

A

Gas gangrene / Myonecrosis

Clostridium perfringens

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8
Q

What are the 2 treatment for myonecrosis?

A

Antibiotic and Surgical debridement of necrotic tissue (Removes infected area)

Hyperbaric oxygen (Kills anaerobe)

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9
Q

NOTE: Myonecrosis can also be cause by:
C. septicum
C. histolyticum
C. noyvi
C. bifermentans

C. prefringens is most often

A
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10
Q

What will happen if gas gangrene is left untreated?

A

Bacteremia

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11
Q

What is the spore component of C. perfringens?

A

Dipicolinic acid

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12
Q

Unique in clostridium perfringens when it comes to Cellular morphology??

A

Boxcar
Large square rods

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13
Q

Unique in clostridium perfringens when it comes to Colonial morphology??

A

Double hemolysis

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14
Q

The alpha toxin in double hemolysis is located in what zone?

A

Outer zone

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15
Q

The Beta toxin in double hemolysis is located in what zone?

A

Inner zone

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16
Q

What are the presumptive test for C. perfringens?

A

Lecithinase test
Nagler reaction
Reverse camp

17
Q

Test that detects alpha toxin Lectihinase C and phospholipase C

A

Lecithinase test

18
Q

What is the media use in lecithinase test?

A

Egg yolk agar

19
Q

What is the positive indicator for lecithinase test

A

Opaque zone around the colony

20
Q

How to prepare nagler reaction?

A

Left side: antitoxin w/ unknown

Right side: W/o antitoxin w/ unknown

21
Q

What is the positive indicator for Nagler reaction?

A

Less to no growth in zone with antitoxin and has zone of precipitation on the area without antitoxin

22
Q

Reverse CAMP is due to?

A

Beta lysin

23
Q

What is the control for Reverse CAMP?

A

Streptococcus agalactiae

24
Q

What is the result for C. perfringens?

A

Reverse CAMP

Bacte Lec [Finals] (59 decks)

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