3.6.4 Inoculation to plated or tubed media

Question 1

Nonselective medium for obligate and facultative anaerobes?

Answer 1

Anaerobic blood and brucella blood agar

Question 2

Selective medium for obligate and G (+) facultative anaerobes

Inhibits G (-) facultative anaerobes

Answer 2

Phenylethyl alcohol
CNA blood agar

Question 3

What is the anaerobic broth?

Answer 3

Thioglycolate broth

Question 4

What is the appearance of strict aerobes in Thioglycolate broth?

Answer 4

Top only turbid

Question 5

What is the appearance of Facultative anaerobes in Thioglycolate broth?

Answer 5

Unevenly Distributed (More in the top)

Question 6

What is the appearance of Aerotolerant anaerobes in Thioglycolate broth?

Answer 6

Evenly distributed

Question 7

What is the appearance of strict anaerobes in Thioglycolate broth?

Answer 7

Turbid on the bottom

Question 8

What is the appearance of Micro aerotolerant in Thioglycolate broth?

Answer 8

Near top

Question 9

Non selective for anaerobes that can be used for gas liquid chromatography

Answer 9

Cooked meat broth

Question 10

Non selective for anaerobes that can be used for GLC and analysis of metabolic end products

Answer 10

Peptone yeast extracts glucose broth

Question 11

Selective medium for Bacteroides and Prevotella?

Answer 11

Kanamycin-Vancomycin laked blood agar

Question 12

Ability to grow in 20% bile and hydrolyze esculin?

Answer 12

Bacteroides bile esculin

Question 13

Selective and differential for Clostridium difficile?

Answer 13

Cycloserin cefoxitin fructose agar

Question 14

For determination of lecithinase and lipase production by Clostridium and Fusobacterium?

Answer 14

Egg-Yolk agar