19b: transcription Flashcards
what kind of an affinity does RNA pol have for a strong promoter?
high affinity
what kind of an affinity does RNA pol have for a weak promoter?
low affinity
what characteristics are important in a promoter? (that make it either strong or weak)
spacing of -35 and -10 region and similarity to the consensus sequence
___________ is important bc the -35/-10 regions need to be on the same __________ of the DNA in order for RNA pol to make contact.
distance; face
how many nucleotide pairs need to be between the middle of the -10 and -35 region?
20 to 22
which strand contains a similar sequence as the mRNA?
partner strand
which strand is complementary to the mRNA?
template strand
in transcription, the strand is read in what direction?
3 to 5
transcription takes place in what direction?
5 to 3
mRNA is made in what direction?
5 to 50
the ___________ is the sequence that contains the ____________ to which a ribosome binds to during ________________
leader sequence; Shine-dalgarno; translation
the ________ of RNA Pol does the transcribing and ___________ is the part of RNA Pol that gives it its specificity for the promoter
core enzyme; sigma factor
the -35 region is _______ of the start site of transcription and the leader sequence is _____________ of the start site of transcription but _____________ of the start codon on the mRNA.
upstream; downstream; upstream
contains more than one gene in the mRNA. Where was this transcribed from?
Polycistronic message; from an operon
what is an operon?
a series of related genes and adjacent genes (located sequentially) under the control of the same promoter
