[LEC/LAB] Antibodies Flashcards
NUMBER OF POLYPEPTIDE UNITS
4
NUMBER OF AMINO ACIDS ON THE HEAVY CHAINS
450
NUMBER OF AMINO ACIDS ON THE LIGHT CHAINS
220
IMMUNOGLOBULIN CLASSES ON THE HEAVY CHAINS
DELTA
ALPHA
GAMMA
EPSILON
MU
IMMUNOGLOBULIN CLASSES ON THE LIGHT CHAINS
KAPPA
LAMBDA
WHICH IMMUNOGLOBULIN CLASSES ARE PRESENT ON ALL LIGHT CHAINS
KAPPA
LAMBDA
FORMER NAME OF IMMUNOGLOBULINS
GAMMA GLOBULINS
WHY IS KAPPA MORE THAN LAMBDA IN THE LIGHT CHAIN
DUE TO GENETIC REARRANGEMENT
LAMBDA STEMS FROM KAPPA
HOLDS LIGHT CHAIN TO HEAVY CHAIN
DISULFIDE BONDS
CONSISTS OF 1 LIGHT CHAIN AND 1/2 HEAVY CHAIN
FAB FRAGMENT
HOW MANY FRAGMENTS MAKE UP 1 AG BINDING SITE
2 FAB FRAGMENTS
MEANING OF FAB
FRAGMENT ANTIGEN BINDING
MEANING OF FC
FRAGMENT CRYSTALLINE
CARBOXY TERMINAL END HALVES OF THE TWO HEAVY CHAINS
FC FRAGMENT
PORTION OF THE ANTIBODY THAT HAS NO AG-BINDING ABILITY
FC PORTION
IG PART THAT IS RESPONSIBLE FOR DETERMINING THE TYPE OF CLASS AND AG-AB REACTION THAT OCCURS
CONSTANT REGION
IG PART THAT DIFFERS ANTIBODY CLASSES FROM EACH OTHER
CONSTANT REGION OF THE HEAVY CHAIN
AMINO TERMINAL END OF THE IG
VARIABLE REGION
PART OF THE IG WHERE AMINO ACID SEQUENCE VARIES
VARIABLE REGION
PART OF THE IG THAT IS RESPONSIBLE FOR ITS SPECIFICITY
VARIABLE REGION
REFERS TO THE NUMBER OF BINDING SITES
VALENCE
FLEXIBLE PORTION OF THE HEAVY CHAIN
HINGE REGION
LOCATION OF THE HINGE REGION
BETWEEN THE FIRST AND SECOND CONSTANT REGIONS
GLYCOPROTEIN THAT SERVES TO LINK IG MONOMERS TOGETHER
J CHAIN
IMMUNOGLOBULINS THAT HAVE A J CHAIN
IGA
IGM
PH AT WHICH SERUM PROTEINS CAN BE SEPARATED
PH 8.6
SLOWEST MOVING PROTEIN
IG
REGION IN WHICH IMMUUNOGLOBULINS APPEAR IN AN ELECTROPHORETIC SET UP
GAMMA BAND
MAIN HUMORAL ELEMENT OF THE ADAPTIVE IMMUNE RESPONSE
IG
FIVE MAJOR CLASSES OF IG
GMADE
HOW MANY VARIABLE REGIONS AND CONSTANT REGIONS DOES A CHAIN HAVE
1 VARIABLE REGION
1 OR MORE CONSTANT REGIONS
SCIENTISTS WHO WORKED ON IGGs
EDELMAN
PORTER
SOLUTION EDELMAN USED TO UNFOLD THE IGG MOLECULE
7M UREA
SOLUTION USED TO BREAK DOWN SULFHYDRYL BONDS
MERCAPTOETHANOL
ENZYME USED TO CLEAVE IGG INTO THREE PIECES
PAPAIN
METHOD USED BY PORTER TO SEPARATE IGG
CARBOXYMETHYL CELLULOSE ION EXCHANGE CHROMATOGRAPHY
CRYSTALLIZATION OF THE FC FRAGMENT OCCURED AT WHAT TEMPERATURE
4C
EFFECTOR FUNCTIONS OF THE FC PORTION
OPSONIZATION
COMPLEMENT FIXATION
ENZYME USED TO CLEAVE IGG AT THE CARBOXY TERMINAL SIDE OF THE INTERCHAIN DISULFIDE BONDS
PAPAIN
DIFFERENCE BETWEEN FC AND FC’
FC1 DISINTEGRATES INTO SEVERAL SMALLER PIECE WHEN CLEAVED
PROTEIN FOUND IN THE URINE OF PATIENTS WITH THIS CLINICAL CONDITION
BENCE JONES PROTEINS
CELLS THAT SECRETE THE BENCE JONES PROTEINS
MALIGNANT PLASMA CELLS
BEHAVIOR OF BENCE JONES PROTEINS WHEN HEATED
60C — PRECIPITATE FROM URINE
80C — REDISSOLVE
PERCENTAGE OF KAPPA CHAINS
60%
[TRUE OR FALSE]
THERE ARE NO FUNCTIONAL DIFFERENCES BETWEEN KAPPA AND LAMBDA CHAINS
TRUE
CHAINS THAT ARE FOUND N ALL FIVE CLASSES OF IG
KAPPA
LAMBDA
MINOR VARIATIONS OF IGG SEQUENCES PRESENT IN SOME INDIVIDUALS BUT NOT OTHERS
ALLOTYPES
UNIQUE AMINO ACID SEQUENCE THAT IS PRESENT ON ALL IG CLASSES IN A GIVEN SPECIES
ISOTYPE
GENETIC MARKERS FOUND ON THE CONSTANT REGION
ALLOTYPES
VARIABLE PORTIONS OF EACH CHAIN THAT ARE UNIQUE TO A SPECIFIC AB MOLECULE
IDIOTYPE
PART OF THE IG THAT CONTAINS THE IDIOTYPE
AMINO TERMINAL ENDS
OF H AND L CHAINS
THE AG RECOGNITION UNIT IS COMPOSED OF WHICH PARTS OF THE IG
AMINO TERMINAL ENDS OF
THE L AND H CHAINS
PROTEIN THAT ALLOWS THE HINGE REGION TO BE FLEXIBLE
PROLINE
IG CLASSES WITH AND WITHOUT THE HINGE REGION
WITH — GDA
WITHOUT — ME
FUNCTIONS OF THE CARBOHYDRATE PORTION
INCREASE SOLUBILITY
PROVIDE PROTECTION AGAINST DEGRADATION
ENHANCE FUNCTIONAL ACTIVITY OF THE FC DOMAINS
REGION THAT IS DIRECTLY IN CONTACT WITH THE AG AND CAN MUTATE TO PRODUCE MORE SPECIFIC AND DIVERSE RESPONSES
HYPERVARIABLE REGION
PREDOMINANT IG IN HUMANS
IGG
(70-75%)
IG WITH THE LONGEST HALF LIFE
IGG
23 DAYS
SUBCLASSES OF IGG
IGG1
IGG2
IGG3
IGG4
DIFFERENTIATING CHARACTERISTIC OF IGG SUBCLASSES
NUMBER AND POSITION OF DISULFIDE BRIDGES