LESSON 9: BACTERIAL GROWTH Flashcards

1
Q

-refers to the increase in number of cells not the size of cells.

A

MICROBIAL GROWTH

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2
Q

-bacteria undergo asexual division to produce 2 daughter cell genitically identical to the parent cell.

A

BINARY FISSION

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3
Q

-the Z-ring is mainly composed of this polymers assemble in the middle of the cell.

A

FtsZ POLYMERS

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4
Q

-it’s the first step of bacterial cytokines.

A

Z-RING

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5
Q

-time required for a bacterium to give rise to 2 daughter cells under optimum conditions.

A

GENERATION TIME

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6
Q

what is generation time o e.coli ?

A

20min, 7 hours can undergo 20 generations

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7
Q

-bacterial counting can be measured in terms of?

A
  1. CELL CONCENTRATION
  2. BIOMASS CONCENTRATION
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8
Q

-it’s a number of viable cell per unit volume

A

CELL CONCENTRATION

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9
Q

-dry weight of cell per unit volume.

A

BIOMASS CONCENTRATION

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10
Q

-it denotes the total number of bacteria whether they are living or deade.

A

TOTAL COUNT

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11
Q

-total number of living or viable bacteria

A

VIABLE COUNT

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12
Q

-viable count can be obtained by 2 method:

A
  1. DILUTION METHOD
  2. PLATING METHOD
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13
Q

-series of sequential dilutions used to reduce a dense
culture of cells to a more usable concentrations.

A

DILUTION METHOD

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14
Q

-number of colonies that grow after a suitable incubation time.

A

PLATING METHOD

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15
Q

-the time between inoculation and beginning of multiplication is known as?

note: no appreciable increase in number
of bacterial cell in this phase

A

LAG PHASE

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16
Q

-This phase is characterized by rapid exponential cell growth of bacteria at their maximum rate.

note: the microbes are sensitive to antibiotics & antimicrobial agents

A

LOG PHASE

17
Q
  • in this phase bacterial growth almost stops
    completely due to depletion of essential nutrients.

note: endospores starting to form in this phase.

A

STAIONARY PHASE

18
Q

-in this phase, the bacterial population declines due to death of cells due to.
1. AUTOLYTIC ENZYME
2. EXHAUSTION OF NUTRIENTS

A

DECLINE PHASE

19
Q

-Is a method utilized for industrial and research purpose that is achieved by using a special device for replenishing nutrients and removing bacterial population continuously so that bacteria growth is not inhibited due to lack of nutrients or due to accumulation of toxic bacterial metabolites

A

CONTINUES CULTURE

20
Q

-minimum and maximum temperature are only what?

A

30 degree celsius

21
Q

-is the lowest temperature at which a species will grow.

A

MINIMUM TEMPERATURE

22
Q

-this temperature is when a species grow best.

A

OPTIMUM TEMPERATURE

23
Q

-is the highest temperature at which a species can grow.

A

MAXIMUM TEMPERATURE

24
Q

-refers to the alkalinity or acidty of a solution.

A

pH

25
Q

-what is the neutral pH

A

6.5-7.5

26
Q

-is the pressure required to stop water/solution from
diffusing through a barrier by?

A

OSMOSIS or OSMOTIC PRESSURE

27
Q
  • by osmosis. Microorganisms require water
    for growth and are made of water, how many percentage?
A

80-90%

28
Q

-when solution outside the cell has higher concentration of solutes than Inside the cell, water is diffused outside of cell and then the cell will SHRINKS

A

HYPERTONIC

29
Q

-When solution outside the cell has lower concentration of
solutes than inside the cell, water is diffused inside of cell and the cell SWELLS

A

HYPOTONIC

30
Q

-when concentrations in two solutions are same, so cell
will neither SWELL nor SHRINK

A

ISOTONIC

31
Q

-important for all organic compounds that make up a living cell;
-also cosist the half dry weight of bacteria

A

CARBON

32
Q

-helps form the amino group in amino acid.

A

NITROGEN

33
Q

-used to synthesize sulfur-containing amino acids and vitamins

A

SULPHUR

34
Q

-important in synthesis of ATP, nucleic acids and
phospholipids of cell membranes.

A

PHOSPHOROUS

35
Q

-minerals that are minimally required by microbes, and
are also essential in enzymatic reaction.

A

TRACE ELEMENTS

36
Q
  • required by aerobic microorganisms, it is toxic
    to anaerobic bacteria like Clostridium tetanus.
A

OXYGEN

37
Q

-are essential organic compounds that cannot
be synthesized by an organism thus must be obtained from the environment

A

ORGANIC GROWTH FACTOR