LESSON 9: BACTERIAL GROWTH Flashcards
-refers to the increase in number of cells not the size of cells.
MICROBIAL GROWTH
-bacteria undergo asexual division to produce 2 daughter cell genitically identical to the parent cell.
BINARY FISSION
-the Z-ring is mainly composed of this polymers assemble in the middle of the cell.
FtsZ POLYMERS
-it’s the first step of bacterial cytokines.
Z-RING
-time required for a bacterium to give rise to 2 daughter cells under optimum conditions.
GENERATION TIME
what is generation time o e.coli ?
20min, 7 hours can undergo 20 generations
-bacterial counting can be measured in terms of?
- CELL CONCENTRATION
- BIOMASS CONCENTRATION
-it’s a number of viable cell per unit volume
CELL CONCENTRATION
-dry weight of cell per unit volume.
BIOMASS CONCENTRATION
-it denotes the total number of bacteria whether they are living or deade.
TOTAL COUNT
-total number of living or viable bacteria
VIABLE COUNT
-viable count can be obtained by 2 method:
- DILUTION METHOD
- PLATING METHOD
-series of sequential dilutions used to reduce a dense
culture of cells to a more usable concentrations.
DILUTION METHOD
-number of colonies that grow after a suitable incubation time.
PLATING METHOD
-the time between inoculation and beginning of multiplication is known as?
note: no appreciable increase in number
of bacterial cell in this phase
LAG PHASE