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MICROBIOLOGY > LESSON 12: CULTURE MEDIA > Flashcards

LESSON 12: CULTURE MEDIA Flashcards

1
Q

Laboratory diagnosis of an infection is usually confirm by:

A
  1. ISOLATING
  2. CULUTURING microorganicm by artificial media
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2
Q

Bacteria and fungi are cultured either by:

A
  1. LIQUID (broth)
  2. SOLID (agar)
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3
Q

-pioneered the use of agar as a base for culture media as suggested by Fannie Eilshemius Hesse

A

ROBERT KOCH

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4
Q

-developed the petri dish.

A

RICHARD PETRI

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5
Q

-a container for solid culture media.

A

PETRI DISH

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6
Q

-developed the pour plate method and was the first to use solid culture media
for culture of bacteria.

A

KOCH

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7
Q

-A nutrient material prepared for the growth of microorganisms in a laboratory is called a?

A

CULTURE MEDIUM

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8
Q
  • When microbes are introduced into a culture medium to initiate growth.
A

INOCULUM

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9
Q

-The microbes that grow and multiply in or on a culture medium are referred to a?

A

CULTURE

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10
Q

-is the process of growing microorganisms in culture by taking bacteria from the infection site by some means of specimen collection and growing them in the artificial environment of the laboratory.

A

CULTIVATION

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11
Q

-a laboratory culture containing a single species of organism.

A

PURE CULTURE

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12
Q

-is prepared from a variety of seaweeds and is now
universally used for preparing solid media.

A

AGAR

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13
Q

-agars melt at about ?

A

95 degree celsius

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14
Q

-agars solidify only when cooled at?

A

42 degree celsius

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15
Q

-accessory growth factors of peptone:

A
  1. riboflavin
  2. nicotinic acid
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16
Q

-. It is a complex mixture of partially digested prote

A

PEPTONE

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17
Q

-It contains a wide range of amino acids, growth factors and inorganic salts.
- is used mainly as a comprehensive source of growth
factors and may be substituted for meat extract in culture media

A

YEAST EXTRACT

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18
Q

-It consists mainly of maltose (about 50%), starch, dextrins and glucose, and contains about 5 percent of proteins and protein breakdown products, and a wide range of mineral salts and growth facto

A

MALT EXTRACT

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19
Q

-hese are used for enriching culture media. Either human or animal blood can BE USED.

A

BLOD SERUM

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20
Q
  • In this type of media nutrients are dissolved in water, and bacterial growth is indicated by a change
    in broth’s appearance from clear to turbid.
A

LIQUID OR BROTH MEDIA

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21
Q

-are made by adding a solidifying agent to
the nutrient.

A

SOLID MEDIA or AGAR

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22
Q

-Petri dish containing the agar is referred to as?

A

AGAR

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23
Q

-are clones of cells originating from a single bacterial cell.

A

CLONES

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24
Q

-the simplest and most common medium used routinely in diagnonistic laboratories.

A

NUTRIENT AGAR by adding 2% of agar to nutrienth broth

25
Q

-for special purposes where agar is added to media in
concentrations that are too low to solidify them

A

SEMI SOLID MEDIA

26
Q

-at this percent it yields a
semisolid medium through which motile, but not non motile, bacteria may spre

A

0.2- 0.5 %

27
Q

-This media include nutrient broth and
peptone water, which form the basis of other media.

A

BASAL MEDIA or SIMPLE MEDIA

28
Q

-is an example of a simple liquid medium that consists
of peptone, meat extract, sodium chloride, and water

A

NUTRIENT BROTH

29
Q

-Addition of 0.5% glucose to nutrient broth makes it?

A

GLUCOSE BROTH

30
Q

-Is an example of a simple solid medium.
- The medium is
used routinely for isolation of many bacteria from clinical specimens.

A

NUTRIENT AGAR

31
Q

-: a media that contain some ingredients of unknown
chemical composition.

A

COMPLEX MEDIA

32
Q

-s a synthetic kind of medium which contains known quantities of all ingredients used but does not contain any animal, yeast, or plant tissue

A

DEFINED MEDIA

33
Q

-is an enriched medium in which nutritionally rich whole
blood supplements constitute the basic nutrients.

A

BLOOD AGAR

34
Q

-is enriched with heat-treated blood (80°C), which turns brown and gives the medium the color for
which it is named.

A

CHOCOLATE AGAR

35
Q

-These are solid media that contain substances that inhibit
the growth of all but a few bacteria but at the same time facilitate isolation of certain bacteria.

A

SELECTIVE MEDIA

36
Q

-selective for the isolation of Vibrio cholerae

A

Thiosulfate citrate bile salt sucrose agar

37
Q

-selective for enteric bacilli, such as Salmonella spp. and Shigella spp..

A

Deoxychocolate citrate agar (DCA)

38
Q

-this medium is intended for Myobacterium tuberculosis.

A

LJ Medium

39
Q

-this 2 medium is selective for Gram-negative bacteria.

A

Hektoen enteric agar (H.E)
& Xylose lysine desoxychocolate agar

40
Q

-this medium is selective for Gram-positive bacteria

A

Mannitol salt agar

41
Q

-selective for certain Gram-negative bacteria, such as Legionella pneumophila

A

Buffered charcoal yeast extract agar

42
Q

-are liquid media that stimulate the growth of certain bacteria or suppress the growth of others for isolation of desired pathogenic bacteria.

A

ENRICHMENT MEDIA

43
Q
  • this bacteria are present in feces, tend to overgrow pathogenic ones in stool specim
A

Escherichia coli.

44
Q

-distinguish one microorganism from another growing on the same media by their growth characteristics.

A

INDICATOR MEDIA

45
Q

-differential for lactose
and sucrose fermentation.

A

EOSIN METHYLENE BLUE

46
Q

-differential for lactose fermentatiuon

A

MacConkey

47
Q

-differential for mannitol fermentation

A

Mannitol salt agar

48
Q

-differential for lac operon mutants for detection of recombinant strains of bacteria for
study in molecular biology.

A

X-gal plates

49
Q

-media are used to maintain the viability of
certain delicate organisms in clinical specimens during their transport to the
laboratory.

A

TRANSPORT MEDIA

50
Q

-what medium is for Neisseria gonorrhoeae

A

Stuart’s transport medium

51
Q

-in microbiology denotes any fermentable substance, such as glucose, sucrose, lactose, and mannitol that is routinely used

A

SUGAR MEDIA

52
Q

-sugar media is contains how many % of sugar?

A

1%

53
Q

-can be used for the short-term storage of bacterial
cultures.

A

REFRIGERATION

54
Q
  • a process in which a pure culture of microbes is
    placed in a suspending liquid and quick-frozen at temperatures ranging from - 50º to-95°C.
A

DEEP FREEZING

55
Q

-a suspension of microbes is quickly frozen at temperatures ranging from -54º to -95ºC, and the water is removed by a high vacuum (sublimation).

A

LYOPHILIZATION

56
Q

-The organisms can be
revived at any time by hydration with a suitable liquid nutrient medium

A

LYOPHILIZATION

57
Q
  • is a common method of preserving strains
A

COLD STORAGE

58
Q

-number of drying methods for drying suspensions of bacteria for preservation purposes have been developed.

A

DRYING METHOD

59
Q
A

MICROBIOLOGY (13 decks)

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