LESSON 12: CULTURE MEDIA

Question 1

Laboratory diagnosis of an infection is usually confirm by:

Answer 1

1. ISOLATING
2. CULUTURING microorganicm by artificial media

Question 2

Bacteria and fungi are cultured either by:

Answer 2

1. LIQUID (broth)
2. SOLID (agar)

Question 3

-pioneered the use of agar as a base for culture media as suggested by Fannie Eilshemius Hesse

Answer 3

ROBERT KOCH

Question 4

-developed the petri dish.

Answer 4

RICHARD PETRI

Question 5

-a container for solid culture media.

Answer 5

PETRI DISH

Question 6

-developed the pour plate method and was the first to use solid culture media
for culture of bacteria.

Answer 6

KOCH

Question 7

-A nutrient material prepared for the growth of microorganisms in a laboratory is called a?

Answer 7

CULTURE MEDIUM

Question 8

• When microbes are introduced into a culture medium to initiate growth.

Answer 8

INOCULUM

Question 9

-The microbes that grow and multiply in or on a culture medium are referred to a?

Answer 9

CULTURE

Question 10

-is the process of growing microorganisms in culture by taking bacteria from the infection site by some means of specimen collection and growing them in the artificial environment of the laboratory.

Answer 10

CULTIVATION

Question 11

-a laboratory culture containing a single species of organism.

Answer 11

PURE CULTURE

Question 12

-is prepared from a variety of seaweeds and is now
universally used for preparing solid media.

Answer 12

AGAR

Question 13

-agars melt at about ?

Answer 13

95 degree celsius

Question 14

-agars solidify only when cooled at?

Answer 14

42 degree celsius

Question 15

-accessory growth factors of peptone:

Answer 15

1. riboflavin
2. nicotinic acid

Question 16

-. It is a complex mixture of partially digested prote

Answer 16

PEPTONE

Question 17

-It contains a wide range of amino acids, growth factors and inorganic salts.
- is used mainly as a comprehensive source of growth
factors and may be substituted for meat extract in culture media

Answer 17

YEAST EXTRACT

Question 18

-It consists mainly of maltose (about 50%), starch, dextrins and glucose, and contains about 5 percent of proteins and protein breakdown products, and a wide range of mineral salts and growth facto

Answer 18

MALT EXTRACT

Question 19

-hese are used for enriching culture media. Either human or animal blood can BE USED.

Answer 19

BLOD SERUM

Question 20

• In this type of media nutrients are dissolved in water, and bacterial growth is indicated by a change
  in broth’s appearance from clear to turbid.

Answer 20

LIQUID OR BROTH MEDIA

Question 21

-are made by adding a solidifying agent to
the nutrient.

Answer 21

SOLID MEDIA or AGAR

Question 22

-Petri dish containing the agar is referred to as?

Answer 22

AGAR

Question 23

-are clones of cells originating from a single bacterial cell.

Answer 23

CLONES

Question 24

-the simplest and most common medium used routinely in diagnonistic laboratories.

Answer 24

NUTRIENT AGAR by adding 2% of agar to nutrienth broth

Question 25

-for special purposes where agar is added to media in concentrations that are too low to solidify them

Answer 25

SEMI SOLID MEDIA

Question 26

-at this percent it yields a semisolid medium through which motile, but not non motile, bacteria may spre

Answer 26

0.2- 0.5 %

Question 27

-This media include nutrient broth and peptone water, which form the basis of other media.

Answer 27

BASAL MEDIA or SIMPLE MEDIA

Question 28

-is an example of a simple liquid medium that consists of peptone, meat extract, sodium chloride, and water

Answer 28

NUTRIENT BROTH

Question 29

-Addition of 0.5% glucose to nutrient broth makes it?

Answer 29

GLUCOSE BROTH

Question 30

-Is an example of a simple solid medium. - The medium is used routinely for isolation of many bacteria from clinical specimens.

Answer 30

NUTRIENT AGAR

Question 31

-: a media that contain some ingredients of unknown chemical composition.

Answer 31

COMPLEX MEDIA

Question 32

-s a synthetic kind of medium which contains known quantities of all ingredients used but does not contain any animal, yeast, or plant tissue

Answer 32

DEFINED MEDIA

Question 33

-is an enriched medium in which nutritionally rich whole blood supplements constitute the basic nutrients.

Answer 33

BLOOD AGAR

Question 34

-is enriched with heat-treated blood (80°C), which turns brown and gives the medium the color for which it is named.

Answer 34

CHOCOLATE AGAR

Question 35

-These are solid media that contain substances that inhibit the growth of all but a few bacteria but at the same time facilitate isolation of certain bacteria.

Answer 35

SELECTIVE MEDIA

Question 36

-selective for the isolation of Vibrio cholerae

Answer 36

Thiosulfate citrate bile salt sucrose agar

Question 37

-selective for enteric bacilli, such as Salmonella spp. and Shigella spp..

Answer 37

Deoxychocolate citrate agar (DCA)

Question 38

-this medium is intended for Myobacterium tuberculosis.

Answer 38

LJ Medium

Question 39

-this 2 medium is selective for Gram-negative bacteria.

Answer 39

Hektoen enteric agar (H.E) & Xylose lysine desoxychocolate agar

Question 40

-this medium is selective for Gram-positive bacteria

Answer 40

Mannitol salt agar

Question 41

-selective for certain Gram-negative bacteria, such as Legionella pneumophila

Answer 41

Buffered charcoal yeast extract agar

Question 42

-are liquid media that stimulate the growth of certain bacteria or suppress the growth of others for isolation of desired pathogenic bacteria.

Answer 42

ENRICHMENT MEDIA

Question 43

- this bacteria are present in feces, tend to overgrow pathogenic ones in stool specim

Answer 43

Escherichia coli.

Question 44

-distinguish one microorganism from another growing on the same media by their growth characteristics.

Answer 44

INDICATOR MEDIA

Question 45

-differential for lactose and sucrose fermentation.

Answer 45

EOSIN METHYLENE BLUE

Question 46

-differential for lactose fermentatiuon

Answer 46

MacConkey

Question 47

-differential for mannitol fermentation

Answer 47

Mannitol salt agar

Question 48

-differential for lac operon mutants for detection of recombinant strains of bacteria for study in molecular biology.

Answer 48

X-gal plates

Question 49

-media are used to maintain the viability of certain delicate organisms in clinical specimens during their transport to the laboratory.

Answer 49

TRANSPORT MEDIA

Question 50

-what medium is for Neisseria gonorrhoeae

Answer 50

Stuart’s transport medium

Question 51

-in microbiology denotes any fermentable substance, such as glucose, sucrose, lactose, and mannitol that is routinely used

Answer 51

SUGAR MEDIA

Question 52

-sugar media is contains how many % of sugar?

Answer 52

1%

Question 53

-can be used for the short-term storage of bacterial cultures.

Answer 53

REFRIGERATION

Question 54

- a process in which a pure culture of microbes is placed in a suspending liquid and quick-frozen at temperatures ranging from - 50º to-95°C.

Answer 54

DEEP FREEZING

Question 55

-a suspension of microbes is quickly frozen at temperatures ranging from -54º to -95ºC, and the water is removed by a high vacuum (sublimation).

Answer 55

LYOPHILIZATION

Question 56

-The organisms can be revived at any time by hydration with a suitable liquid nutrient medium

Answer 56

LYOPHILIZATION

Question 57

- is a common method of preserving strains

Answer 57

COLD STORAGE

Question 58

-number of drying methods for drying suspensions of bacteria for preservation purposes have been developed.

Answer 58

DRYING METHOD