LABORATORY LESSON 1

Question 1

Students in microbiology must understand and follow the rules in the lab. Student should be:

Answer 1

1. familiar with the location
2. use of the safety equipment in the laboratory
3. be aware of all exits from the room.

Question 2

laboratories classified as:

Answer 2

1.biosafety level I (BSL)
2. biosafetY leve (BSL2)

Question 3

organisms used in BL2
laboratory are classified as:

Answer 3

CAUSATIVE AGENTS

Question 4

• is the application of safety precautions that reduce a laboratorian’s risk
  of exposure to a potentially infectious microbe and limit contamination of the work
  environment and, ultimately, the community.

Answer 4

BIOSAFETY

Question 5

-organisms are well-characterized strains of microorganisms not known to cause disease in healthy human adults. Precautions in this lab include general laboratory safety.

Answer 5

BSL1

Question 6

example of organism in bsl 1:

Answer 6

Escherichia coli,
Staphylococcus xylosus,
Bacillus mega

Question 7

organisms are moderate-risk microorganisms associated with less serious human diseases whose potential for transmission is limited and a proven treatment for the disease exist.

Answer 7

BSL2

Question 8

organisms are high-risk microorganisms with a true potential for infection by aerosols and in which the resulting disease may have serious or lethal consequences

Answer 8

BSL 3

Question 9

example of organism in BSL 3:

Answer 9

Mycobacterium tuberculosis
Foot-and-mouth disease virus,
Bacillus

Question 10

organisms are easily transmitted, very-high risk
microorganisms which cause life-threatening diseases for which there is no vaccine or
therapy.

Answer 10

BSL 4

Question 11

“space suits” with an external oxygen supply, and precautions such as chemical showers must
be taken before exiting in this bsl:

Answer 11

BSL 4

Question 12

Example of BSL4

Answer 12

Ebola virus
Marburg virus
Lassa fe
Avian flu virus

Question 13

Steps of risk management process:

Answer 13

1. IDENTIFY HAZARAD AND RISK
2. EVALUATE THE RISK
3. IMPLEMENT A MITOGATION PLAN AS NEEDED
4. EVALUATE EFFECTIVENESS OF CONTROL

Question 14

starting point for the laboratory investigation is the taking of,

Answer 14

SAMPLES

Question 15

between samples. The samples should
be:

Answer 15

1. carefully packaged
   2.labeled
2. transmitted to the laboratory by the fastest
   4.practicable method, with the appropriate temperature cont

Question 16

Sample collection from live animals:

Answer 16

1. BLOOD
2. SKIN
3. GENITAL TRACT & SEMEN
4. EYE
5. NASAL DISCHARGE
6. MILK

Question 17

types of sample:

Answer 17

1. SAMPLE COLLECTION FOR LIVE ANIMALS
2. SAMPLE COLLECTION AT POST MORTEM
3. ENVIRONMENTAL & FEEDING SAMPLING
4. INFORMATION TO BE SENT W/ SAMPLE
5. PRESERVATION OF SAMPLE FOR PROLONGED STORAGE

Question 18

to examine microorganisms which can’t
be seen by naked eye

Answer 18

MICROSCOPE

Question 19

to heat or boil solution in laboratory

Answer 19

BUNSEN BURNER

Question 20

2 equipment use to sterilize the equipment medias and other solutions

Answer 20

1. AUTOCLAVE
2. PRESSURE COOKER

Question 21

to preserve the samples, media, reagents
and other specimen

Answer 21

REFRIGERATOR

Question 22

creates a rotating magnetic field

Answer 22

MAGNETIC STRRING PLATE

Question 23

used for bacterial or fungal cultures

Answer 23

INCUBATORS

Question 24

to rescue the exposure of the operator
and the lab contamination

Answer 24

SAFETY CABINET

Question 25

used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms

Answer 25

INOCULATING LOOP

Question 26

to act as a supporting container to hold the culture medium.

Answer 26

PETRI DISH

Question 27

2 types of test tube:

Answer 27

1. TEST TUBE 2. TEST TUBE RACK

Question 28

to measure and prepare media

Answer 28

FLASK

Question 29

to measure and transfer the solution

Answer 29

BEAKER

Question 30

to measure the weight of an specimen

Answer 30

WEIGHING SCALE

Question 31

to transfer quantified volume of specimen or solution.

Answer 31

PIPETTOR

Question 32

pipette tips are used as:

Answer 32

Almost all the pipettes require pipette tips for performing their intended work

Question 33

Used to heat medium gently (to around 45-55℃) during media preparation

Answer 33

WATER BATH

Question 34

partner of GLASS SLIDES?

Answer 34

COVER SLIPS

Question 35

this bottles protect chemicals sensitive to light and ultraviolet rays.

Answer 35

AMBER BOTTLES

Question 36

for storage of media, regeants and other materials

Answer 36

CUPBOARDS

Question 37

Storage cabinet for personal protective equipment

Answer 37

PPE's