LABORATORT LESSON 2

Question 1

refers to the removal of dirt and impurities, including germs, from surfaces. Cleaning alone does not kill germs. But by removing the germs, it decreases their number
and therefore any risk of spreading infection.

Answer 1

CLEANING

Question 2

Cleasing is achieved with:

Answer 2

SOAP
WATER

Question 3

works by using chemicals to destroy, inhibit growth, or removal
microbes on surfaces. But killing germs remaining on a surface after cleaning further
reduces any risk of spreading infection

Answer 3

DISINFECTANT

Question 4

means the complete destruction of all the micro-organisms including
spores, from an object or environment. There are two types of sterilization commonly used
in our laboratory.

Answer 4

STERILIZATION

Question 5

2 types of sterilization :

Answer 5

1. DRY HEAT STERILIZATION
2. MOIST HEAT STERILIZATION

Question 6

is using the principle of steam under pressure through
autoclave or pressure cooker.

Answer 6

MOIST HEAT STERILIZATION

Question 7

is also used to
keep the autoclaved media at around 45-50℃ before dispensing it to the Petri dish.

Answer 7

WATER BATH

Question 8

method is important in that it does not affect the glass surface; do not
corrode metals, able to sterilize powders, oil and other viscous substances without getting
moist or evaporated

Answer 8

DRY HEAT METHOD