Transcription Flashcards
Where does transcription take place in P and E
P - Cytoplasm
E - Nucleus
Name given to the stretch of DNA that is transcribed into RNA
Transcription unit
Name given to specific sequence that marks beginning of transcription
Promoter region
What are the 3 stages of transcription
Binding and initation
Elongation
Termination
Term used for the binding of RNA polymerase to the template strand
Template binding
Which strand is the antisense strand
Template strand
Where are the promoters found
Upstream from the point of initial transcription
What are used instead of primers during transcription and what is formed when RNA polymerase binds to the template strand
Transcription factors - Transcription initiation complex
What is the 4 base sequence used as a promoter in E
TATA
Why is transcription asymmetric
Only one strand serves as a template strand
Role of RNA polymerase in elongation
untwisting of double helix and assembling the RNA polynucleotide in the 5’ 3’ direction
How does termination differ in P and E
When the terminator sequence is transcribed in P the RNA polymerase detaches from the DNA and releases the mRNA immediately whereas in E detachment occurs 10-35 nucelotides downstream
What needs to occur before translation and exiting of mRNA from the nucelus
Post-transcriptional processing
Which end does capping (G) and tailing (A) occur and what are the 3 reasons for it
Capping - 5’
Tailing - 3’
Prevents degredation against hydrolytic enzymes
Facilitates exit of mRNA from nucleus
Facilitates ribosomal attachment
What does splicing remove and why does it occur
Introns - they are non-coding stretches of nucleotides
