1.4 Proteins: Enzymes Flashcards

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1
Q

What is the role of an enzyme?

A

Reduces the activation energy to catalyse a chemical reaction

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2
Q

What biological molecule is an enzyme?

A

Protein

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3
Q

What are the monomers of an enzyme?

A

Amino acids

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4
Q

What test can be done for proteins?

A

Biurets test

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5
Q

What is a positive result for the Bieurets test?

A

Blue -> Lilac

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6
Q

What is the induced fit model?

A

Substrate and active site similar but not complementary
When substrate enters active site, it changes the shape of the active site slightly to make it complementary
Puts pressure/tension/breaks bonds within the substrate
Lowers the activation energy of the reaction

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7
Q

What is required for a reactions activation energy to be reduced?

A

Constant movement of both enzyme and substrate

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8
Q

Describe the effect of increasing temperature on enzyme activity.

A

Increases up to the optimum and then decreases

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9
Q

Explain how temperature effects enzyme activity.

A

More kinetic energy means more successful collisons and more ES complexes
After optimum, enzyme active site begins to denature

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10
Q

Describe the effect of pH on enzyme activity.

A

Each enzyme has its own optimum pH
Enzymes denature too far from optimum

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11
Q

How does increasing enzyme concentration affect rate of reaction?

A

As concentration increases so does the rate of reaction
More active sites are available
Same amount of products are made as there is the same amount of substrate

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