17.3 Whole-genome sequenceing

Question 1

Whole-genome sequencing

Answer 1

process that determines an
entire genome’s DNA sequence

Question 2

deoxynucleotide (dNTP)

Answer 2

individual DNA monomer (single unit)

Question 3

dideoxynucleotides (ddNTPs)

Answer 3

individual DNA monomer that is
missing a hydroxyl group (–OH)

Question 4

shotgun sequencing

Answer 4

method used to sequence multiple
DNA fragments to generate the sequence of a large piece of DNA

Question 5

contig

Answer 5

larger sequence of DNA assembled from
overlapping shorter sequences

Question 7

next-generation sequencing

Answer 7

group of automated
techniques for rapid DNA sequencing

Question 8

model organism

Answer 8

species that researchers study and use as
a model to understand the biological processes in other
species represented by the model organism

Question 9

genome annotation

Answer 9

process of attaching biological
information to gene sequences

Question 10

DNA microarrays

Answer 10

method to detect gene expression by
analyzing many DNA fragments that are fixed to a glass slide or a silicon chip to identify active genes and identify
sequences