2. Basic Clinical Mycology (I) Flashcards Preview

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Flashcards in 2. Basic Clinical Mycology (I) Deck (46):
1

Specimens for fungi ID can be refrigerated at

4° C

2

Temp of blood and CSF specimens for fungi ID

30-37°C

3

Temp for dermatologic specimens for fungi ID

15-30°C

4

Container for dermatologic specimens

Dry container

5

Biosafety hoods used in the mycology laboratory

Class 2 or 3

6

Type of disinfectant in the mycology laboratory

Phenol-based

7

3 methods for specimen processing

Direct inoculation
Concentration
Minced (homogenized)

8

Specimen processing method; adding several drops of specimen to media

Direct inoculation

9

Specimen processing method; the specimen is streaked if the media is solid

Direct iniculation

10

Specimens for direct inoculation

Bronchial brush/wash
Aspirates
CSF
Swabs
Body fluids
Hairs
Scrapings

11

Specimen processing method; large volumes can be concentratd by centrifugation

Concentration

12

Specimen for centrifugation

Body fluids
CSF
Urine

13

Specimen processing method; some solids must be "destroyed" to expose a buried pathogen to the media

Minced (homogenized)

14

Specimen for mincing (homogenizing)

Nails
Tissues
Biopsies

15

Primary culture media for fungi

Test tubes

16

Culture media for subculture of fungi

Petri dishes

17

Most common media for fungi

Sabouraud dextrose agar (SDA)

18

SDA with less glucose for growth of Blastomyces dermatitidis

Emmon's modification

19

Fungi that grows on Emmon's modification of SDA

Blastomyces dermatitidis

20

Inhibitory substances that may be present in fungal culture media

Chloramphenicol
Gentamicin
Cycloheximide

21

Media for selective recovery of dimorphs and dermatophytes

Mycosel and mycobiotic

22

Composition of mycosel and mycobiotic

SDA + chloramphenicol + cycloheximide

23

Fungi recovered using Mycosel and mycobiotic

Dimorphs
Dermatophytes

24

Enriched media enhanced for recovery of C. neoformans and dimorphic transitions Sporothrix and Paracoccidioides

Brain heart infusion (BHI) agar

25

BHI agar modification for Zygomycetes

Broth + penicillin

26

Fungi recovered from BHI agar (+ broth and penicillin modification)

C. neoformans
Sporothrix (dimorphic transition)
Paracoccidioides (dimorphic transition)
Zygomycetes

27

Media for C. neoformans in contaminated specimen

BHI + gentamicin + chloramphenicol

28

Fungi recovered from BHI + gentamicin + chloramphenicol

C. neoformans in contaminated specimen

29

Enriched medium for Cryptococcus spp. and thermally dimorphic fungi

Sabouraud-brain heart infusion agar (SABHI)

30

Fungi recovered from SABHI

- Cryptococcus spp.
- Thermally dimorphic fungi

31

Selective and differential media for presumptive ID of Candida from primary plates

CHROMagar Candida

32

Light to medium green on CHROMagar

Candida albicans

33

Light blue to metallic-blue on CHROMagar

Candida tropicalis

34

Loght rose with a whitish border on CHROMagar

Candida krusei

35

Media that contains inorganic salts, chloramphenicol, and gentamicin that inhibits bacteria

Inhibitory mold agar (IMA)

36

Fungi recovered for IMA

Cryptococcus spp.

37

Media for dermatophytes from heavily contaminated specimens (pink-to-red color change)

Dermatophyte test medium

38

Media for subculture

Potato dextrose agar (PDA)
Potato flake agar (PFA)

39

Media for yeast morphology

Cornmeal agar

40

Media to differentiate yeast morphology

Cornmeal agar

41

Incubation temp for obligate filamentous fungi

25 or 37°C

42

Incubation temp for dimorphic fungi

25 and 37°C

43

Incubation temp for yeasts

25 or 37°C

44

Incubation period of media

3-4 weeks

45

Media recommended for promoting sporulation

Cornmeal agar

46

Specimen for fungi pathogen must be processed within

2 hours