9. Dimorphic Fungi Flashcards Preview

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Flashcards in 9. Dimorphic Fungi Deck (45):
1

Primary infection of dimorphic fungi

Pulmonary

2

How are tissue specimens processed?

Minced

3

How are pleural fluid and CSF specimens processed?

Concentrated

4

How are mucus and pus specimens processed?

Mucolytic agent

5

Easiest and quickest method for microscopic examination for dimorphic fungi

KOH preparation (+ Calcofluor)

6

Stains used for in situ staining

- H&E
- PAS
- GMS

7

Culture media for primary isolation of dimorphic fungi

SDA
SABHI
BHI

8

Incubation temp of media for primary isolation of dimorphic fungi

30°C

9

Media used for subculture for dimorphic fungi

PDA

10

MOT of dimophic fungi

Respiratory route

11

Immune response to dimorphic fungi

Cellular immune response

12

True/False. Dimorphic fungi are not strong antigens.

True

13

Explain dimorphic fungi antibody detection

Positive: exposure (not necessarily infection)

AIDS patients will be negative

14

What is detected in the expantigen test for dimorphic fungi?

Cell free extracts of the fungus

15

Used to react with known antisera in the exoantigen test for dimorphic fungi

Material extracted from mold phase

16

Causative agent of Gilchrist's disease

Blastomyces dermatitidis

17

Disease caused by B. dermatitidis

Blastomycosis/Gilchrist's disease

18

2 types Blastomycosis

- chronic cutaneous blastomycosis
- systemic blastomycosis

19

Most common manifestations of systemic blastomycosis

- bone lesions
- osteomyelitis

20

After how many days will yeast forms of B. dermatitidis appear on enriched media?

After 10-15 days

21

Associated with Valley fever

Coccidioides immitis

22

The most virulent of all agents of human mycoses

Coccidioides immitis

23

Disease caused by C. immitis

Primary pulmonary coccidiodomycosis

24

After how many days will C. immitis appear on SDA/SABHI?

3-5 days

25

Arthroconidia of C. immitis appear on SDA/SABHI after

7-10 days

26

Has whote and cottony colonies (cobwebs)

C. immitis

27

Has barrel-shaped arthroconidia

C. immitis

28

Associated with disjuncture cells

C. immitis

29

A soil saprobe with high nitrogen content

Histoplasma capsulatum

30

Associated with Spelunker's disease

H. capsulatum

31

Disease caused bu H. capsulatum

Histoplasmosis

32

Cell where H. capsulatum organisms can be found

Inside macrophages (histiocytes)

33

After how many days will the yeast form of H. capsulatum appear?

After 10-15 days

34

Has macroconidia that become tuberculate with age

H. capsulatum

35

A soil saprobe of acid soil

Paracoccidioides brasiliensis

36

Disease caused by P. brasiliensis

Paracoccidioidomycosis

37

Mold colony of P. brasiliensis mature in

2-3 weeks

38

Has a mariner wheel-shaped yeast form

P. brasiliensis

39

Associated with Rose gardener disease

Sporothrix schenckii

40

Disease caused by S. schenckii

Sporotrichosis

41

Mold form of S. schenckii develops in

3-5 days

42

Has hyphae frequently found as ropes

S. schenckii

43

Has conidiophores that produce multiple conidia in flowerets arrangement

S. schenckii

44

Has a yeast form that is cigar-shaped

S. schenckii

45

Enumerate the thermal dimorphs

- Blastomyces dermatitidis
- Coccidioides immitis
- Histoplasma capsulatum
- Paracoccidioides brasiliensis
- Sporothrix schenckii
- Penicillium marneffei