9. Dimorphic Fungi Flashcards
(45 cards)
Primary infection of dimorphic fungi
Pulmonary
How are tissue specimens processed?
Minced
How are pleural fluid and CSF specimens processed?
Concentrated
How are mucus and pus specimens processed?
Mucolytic agent
Easiest and quickest method for microscopic examination for dimorphic fungi
KOH preparation (+ Calcofluor)
Stains used for in situ staining
- H&E
- PAS
- GMS
Culture media for primary isolation of dimorphic fungi
SDA
SABHI
BHI
Incubation temp of media for primary isolation of dimorphic fungi
30°C
Media used for subculture for dimorphic fungi
PDA
MOT of dimophic fungi
Respiratory route
Immune response to dimorphic fungi
Cellular immune response
True/False. Dimorphic fungi are not strong antigens.
True
Explain dimorphic fungi antibody detection
Positive: exposure (not necessarily infection)
AIDS patients will be negative
What is detected in the expantigen test for dimorphic fungi?
Cell free extracts of the fungus
Used to react with known antisera in the exoantigen test for dimorphic fungi
Material extracted from mold phase
Causative agent of Gilchrist’s disease
Blastomyces dermatitidis
Disease caused by B. dermatitidis
Blastomycosis/Gilchrist’s disease
2 types Blastomycosis
- chronic cutaneous blastomycosis
- systemic blastomycosis
Most common manifestations of systemic blastomycosis
- bone lesions
- osteomyelitis
After how many days will yeast forms of B. dermatitidis appear on enriched media?
After 10-15 days
Associated with Valley fever
Coccidioides immitis
The most virulent of all agents of human mycoses
Coccidioides immitis
Disease caused by C. immitis
Primary pulmonary coccidiodomycosis
After how many days will C. immitis appear on SDA/SABHI?
3-5 days
