6. Subcutaneous Mycoses

Question 1

3 types of conidiation of dematiaceous fungi

Answer 1

Cladosporium type
Phialophora type
Rhinocladiella type

Question 2

Conidiation of dematiaceous fungi; resembles a tree, in which conidiophore is the trunk and branched chains of conidia form the branches

Answer 2

Cladosporium type

Question 3

Conidiation of dematiaceous fungi; short conidiophores + phialide, vase shaped, conidia extruded from phialide and then cluster

Answer 3

Phialophora type

Question 4

Conidiation of dematiaceous fungi; stalked conidiophores that become knobbly as conidia produced sequentially until a Cladosporium type of conidiation is reached

Answer 4

Rhinocladiella type

Question 5

Specimens for subcutaneous fungi ID is collected by

Answer 5

Aspiration

Question 6

Media for subcutaenous fungi ID

Answer 6

SDA

PDA (subculture)

Question 7

A chronic granulomatous disease of feet (lower extremeties)

Answer 7

Mycetoma (Madura foot/Maduromycosis)

Question 8

2 types of mycetoma

Answer 8

Eumycotic

Actinomycotic

Question 9

Mycetoma granule; mycelium with hyphae 1 μm in diameter

Answer 9

Actinomycotic granule

Question 10

Mycetoma granule; wide hyphae (2-4 μm) terminating in chlamydoconidia

Answer 10

Eumycotic granules

Question 11

Verrucoid (wartlike) lesions on feet, legs, hands, and buttocks

Answer 11

Chromoblastomycosis

Question 12

Microscopic:

Single-celled or clusters of single-cells
Dark pigment

Answer 12

Chromoblastomycosis

Question 13

Culture temp and time for Chromoblastomycosis

Answer 13

6 weeks at RT

Question 14

Subcutaneous mycosis that can have a systemic spread

Answer 14

Phaeohyphomycosis

Question 15

Subcutaneous mycosis that uses the types of conidiation as a guide for ID

Answer 15

Chromoblastomycosis

Question 16

Subcutaneous fungi that causes mycetoma

Answer 16

Pseudoallescheria boydii
Exophiala jeanselmei
Acremonium spp.

PEA

Question 17

Subcutaneous fungi that causes Chromoblastomycosis

Answer 17

• Cladophialophora carrionii (Cladosporium type)
• Fonsecaea pedrosoi (Cladosporium, Phialophora, Rhinocladiella type)
• Fonsecaea compacta (Cladosporium type)
• Phialophora verrucosa (Phialophora type)

Question 18

Subcutaneous fungi that causes Phaeohyphomycosis

Answer 18

Phialophora verrucosa
Fonsecaea pedrosoi
Exophiala jeanselmei
Wangiella dermatitidis

Question 19

How many days will Cladophialophora carrionii grow?

Answer 19

30 days

Question 20

Acquired when not wearing shoes, trauma

Answer 20

Cladophialophora carrionii

Question 21

Colonies are grey-green to black on surface and cottony on reverse side within 30 days

Answer 21

Cladophialophora carrionii

Question 22

Gray-green to black, cottony colony within 21 days

Answer 22

Fonsecaea pedrosoi

Question 23

How many days will Fonsecaea pedrosoi grow?

Answer 23

21 days

Question 24

Olive-green to black, velvety colonies

Answer 24

Phialophora verrucosa

Question 25

Name for alternate asexual stage of Pseudoallescheria boydii

Answer 25

Scedosporium apiospermum

Question 26

Macroscopic: - rapid grower - white to brownish-gray, fluffy colonies

Answer 26

Pseudoallescheria boydii

Question 27

Microscopic: - hyaline, septate hyphae - single anelloconidia produced on an anellophore (conidiophore)

Answer 27

Pseudoallescheria boydii

Question 28

Young cultures: black yeasts | Mature cultures: velvety colonies

Answer 28

Exophiala jeanselmei

Question 29

Sticklike coniodiophores with clustered conidia

Answer 29

Exophiala jeanselmei

Question 30

Optimum growth temp of Wangiella dermatitidis

Answer 30

40-42°C

Question 31

Macroscopic: - initially black yeast - after 10 days, olive-gray to black velvety or glabrous colony

Answer 31

Wangiella dermatitidis

Question 32

Microscopic: - pigmented, septate hyphae - conidiophores are indistinguishable from vegetative hyphae, except that conidia are clustered at ends

Answer 32

Wangiella dermatitidis

Question 33

Has similar morphology to E. jeanselmei

Answer 33

Wangiella dermatitidis

Question 34

Etiologic agent of mycetomas, corneal infections, and nail infections

Answer 34

Acremonium spp.

Question 35

4 major infections under subcutaneous mycoses

Answer 35

Mycetoma Chromoblastomycosis Phaeohyphomycosis Sporotrichosis