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Elsevier Mycology (RVSP) > 2. Basic Clinical Mycology (II) > Flashcards

2. Basic Clinical Mycology (II) Flashcards

(50 cards)

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1
Q

Percent of KOH used in KOH preparation

A

15% KOH

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2
Q

Added to KOH preparation that binds to cell wall and fluoresces blue-white under ultraviolet light

A

Calcofluor white

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3
Q

Used to visualize the capsule of C. neoformans in CSF; negative staining

A

India ink stain

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4
Q

India ink stain has been replaced with

A

Cryptococcal antigen test

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5
Q

Fungi stained with Giemsa, Wright-Giemsa

A

Histoplasmosis capsulatum (intracellular)

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6
Q

Color of fungi in H&E

A

Pink to pinkish-blue

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7
Q

Fungi stained with Meyer’s mucicarmine

A

C. neoformans

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8
Q

Color of C. neoformans in Meyer’s mucicarmine

A

Rose red

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9
Q

Stain for C. neoformans (rose red)

A

Meyer’s mucicarmine

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10
Q

Color of fungi in Gomori methenamine silver (GMS)

A

Black

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11
Q

Color of fungi in Papanicolaou stain

A

Pink to blue

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12
Q

Color of fungi in Periodic acid-Schiff (PAS)

A

Red or purple

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13
Q

Red or purple color

A

PAS

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14
Q

Pink to blue

A

Papanicolaou stain

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15
Q

Black

A

GMS

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16
Q

Rose red

A

Meyer’s mucicarmine

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17
Q

Pink to pinkish-blue

A

H&E

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18
Q

Growth period of yeasts

A

2-3 days

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19
Q

Growth period of molds

A

Rapid:

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20
Q

Dictates the texture of fungi colonies

A

Aerial hyphae

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21
Q

Leathery, waxy texture

22
Q

Suede, plush texture

23
Q

Texture that looks like Staphylococcus

24
Q

Fluffy texture

25
Powdery texture
Granular
26
Radial grooves, "folded" topography
Rugose
27
Central depression and raised edge topography
Crateriform
28
Rough knobs topography
Verrucous
29
Brainlike topography
Cerebriform
30
Methods for examination of molds
- tease/cut preparation - scotch tape preparation - slide culture
31
Examination of molds; organism removed directly from culture plate using dissecting needle then placed on a slide
Tease/cut preparation
32
Examination of molds; a scotch tape is used to transfer aerial hyphae from a colony to a microscope slide
Scotch tape preparation
33
Examination of molds; organism is subcultured on a piece of agar then covered with coverslip, the organism grows onto coverslip
Slide culture
34
Best method for examination of molds;
Slide culture
35
Used to stain and preserve fungal elements in culture isolate
Lactophenol cotton blue (LPCB)
36
Stain used for examination of molds
Lactophenol cotton blue (LPCB)
37
3 methods for dermatophyte ID
Hair perforation Urease test Trichophyton agars Rice grain growth
38
How to do hair perforation for dermatophyte ID?
- 5-10 mm sterile hair is floated on sterile water and yeast extract - conidia or hyphae are inoculated onto water surface - remove hair shafts and observe in LPCB weekly for 1 month
39
Positive for hair perforation for dermatophyte ID
Trichophyton mentagrophytes
40
Negative for hair perforation in dermatophyte ID
Trichophyton rubrum
41
Observation time for hair perforation for dermatophyte ID
Weekly for 1 month
42
Observation time for urease test for dermatophyte ID
5 days at RT
43
Positive for urease test for dermatophyte ID
T. mentagrophytes
44
Negative/weak for urease test for dermatophyte ID
T. rubrum
45
Trichophyton agars used in labs
- Trichophyton agar 1 (without thiamine) | - Trichophyton agar 2 (with thiamine)
46
Observation time for Trichophyton agars for dermatophyte ID
10-14 days
47
Media for rice grain growth for dermatophyte ID
Sterile, nonfortified rice grain media
48
Observation time for rice grain growth for dermatophyte ID
10 days
49
Fungi differentiated in rice grain growth for dermatophyte ID
Microsporum canis | Microsporum audouinii
50
Used to dissolve nonfungal materials in skin, nail, hair, fluids, exudates, and biopsy specimens
KOH preparation

Elsevier Mycology (RVSP) (13 decks)

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