2. Basic Clinical Mycology (II) Flashcards Preview

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Flashcards in 2. Basic Clinical Mycology (II) Deck (50):
1

Percent of KOH used in KOH preparation

15% KOH

2

Added to KOH preparation that binds to cell wall and fluoresces blue-white under ultraviolet light

Calcofluor white

3

Used to visualize the capsule of C. neoformans in CSF; negative staining

India ink stain

4

India ink stain has been replaced with

Cryptococcal antigen test

5

Fungi stained with Giemsa, Wright-Giemsa

Histoplasmosis capsulatum (intracellular)

6

Color of fungi in H&E

Pink to pinkish-blue

7

Fungi stained with Meyer's mucicarmine

C. neoformans

8

Color of C. neoformans in Meyer's mucicarmine

Rose red

9

Stain for C. neoformans (rose red)

Meyer's mucicarmine

10

Color of fungi in Gomori methenamine silver (GMS)

Black

11

Color of fungi in Papanicolaou stain

Pink to blue

12

Color of fungi in Periodic acid-Schiff (PAS)

Red or purple

13

Red or purple color

PAS

14

Pink to blue

Papanicolaou stain

15

Black

GMS

16

Rose red

Meyer's mucicarmine

17

Pink to pinkish-blue

H&E

18

Growth period of yeasts

2-3 days

19

Growth period of molds

Rapid:

20

Dictates the texture of fungi colonies

Aerial hyphae

21

Leathery, waxy texture

Glabrous

22

Suede, plush texture

Velvety

23

Texture that looks like Staphylococcus

Yeastlike

24

Fluffy texture

Cottony

25

Powdery texture

Granular

26

Radial grooves, "folded" topography

Rugose

27

Central depression and raised edge topography

Crateriform

28

Rough knobs topography

Verrucous

29

Brainlike topography

Cerebriform

30

Methods for examination of molds

- tease/cut preparation
- scotch tape preparation
- slide culture

31

Examination of molds; organism removed directly from culture plate using dissecting needle then placed on a slide

Tease/cut preparation

32

Examination of molds; a scotch tape is used to transfer aerial hyphae from a colony to a microscope slide

Scotch tape preparation

33

Examination of molds; organism is subcultured on a piece of agar then covered with coverslip, the organism grows onto coverslip

Slide culture

34

Best method for examination of molds;

Slide culture

35

Used to stain and preserve fungal elements in culture isolate

Lactophenol cotton blue (LPCB)

36

Stain used for examination of molds

Lactophenol cotton blue (LPCB)

37

3 methods for dermatophyte ID

Hair perforation
Urease test
Trichophyton agars
Rice grain growth

38

How to do hair perforation for dermatophyte ID?

- 5-10 mm sterile hair is floated on sterile water and yeast extract
- conidia or hyphae are inoculated onto water surface
- remove hair shafts and observe in LPCB weekly for 1 month

39

Positive for hair perforation for dermatophyte ID

Trichophyton mentagrophytes

40

Negative for hair perforation in dermatophyte ID

Trichophyton rubrum

41

Observation time for hair perforation for dermatophyte ID

Weekly for 1 month

42

Observation time for urease test for dermatophyte ID

5 days at RT

43

Positive for urease test for dermatophyte ID

T. mentagrophytes

44

Negative/weak for urease test for dermatophyte ID

T. rubrum

45

Trichophyton agars used in labs

- Trichophyton agar 1 (without thiamine)
- Trichophyton agar 2 (with thiamine)

46

Observation time for Trichophyton agars for dermatophyte ID

10-14 days

47

Media for rice grain growth for dermatophyte ID

Sterile, nonfortified rice grain media

48

Observation time for rice grain growth for dermatophyte ID

10 days

49

Fungi differentiated in rice grain growth for dermatophyte ID

Microsporum canis
Microsporum audouinii

50

Used to dissolve nonfungal materials in skin, nail, hair, fluids, exudates, and biopsy specimens

KOH preparation