Lecture 2 Cell Culture Flashcards
1
Q
What are the requirements for cell culture
A
surface to grow
food
vitamins/essential amino acids
growth factors (serum)
2
Q
what are primary cell cultures
A
growing cells directly from a tissue
3
Q
what are proteases
A
enzymes that cut proteins
4
Q
what is EDTA
A
chelates that holds cells together
5
Q
what are immortalized cells
A
cells that keep growing forever
6
Q
what is contact inhibition
A
cells stop growing because they are in contact with each other
7
Q
what is confluency
A
% of the dish covered by cells
8
Q
what is passaging
A
moving cells to a new plate
9
Q
what is a cell line/clonal population
A
identical cells from the primary cell
10
Q
what is a callus
A
a cultured mass of undifferentiated plant cells
