Transcription Flashcards

1
Q

what does RNA contain instead of a deoxyribose

A

ribose

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2
Q

what is the structure of RNA

A

single stranged

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3
Q

what is transcription catalyzed by

A

RNA polyermase

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4
Q

what does RNA polyermase do

A

moves along templant DNA and unwinds it. Also catalyzes formation of phosphdiester bonds increasing the length of RNA polyer

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5
Q

difference between RNA polymerase and DNA polymerase

A

RNA polymerases initate RNA synthesis without a primer. more error prone

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6
Q

RNA polyermase used in mRNAs

A

POl II

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7
Q

RNA polyermase unsed in rRNAs

A

Pol I mainly, some Pol III

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8
Q

RNA polyermase used in tRNAs

A

pol III

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9
Q

RNA polyermase used in snoRNAs

A

pol II

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10
Q

RNA polyermase used in other non-coding RNAs

A

pol III and II

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11
Q

function of rRNA

A

components fo ribosome that are required for protein synthesis

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12
Q

function of tRNA

A

serve as adapters between mRNA and amino acids during protein synthesis

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13
Q

function of snRNAs

A

serve a number o ffunction in the nucleus, including pre-mRNA splicing

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14
Q

function of snoRNAs

A

process and modify rRNAs

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15
Q

what is the most abundent RNA in the cell

A

rRNA

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16
Q

how is tRNA molecules synthesized

A

larger precurosor molecules are trimmed and chemically modified

17
Q

how does pol II know where to start

A

promoter region of DNA

18
Q

what is common in promoter regions

A

consensus DNA

19
Q

consensus sequence

A

sequence is similiar but not necessarily identifcal

20
Q

examples of consensus sequres

A

TATA box, CAAT box, GC region

21
Q

what consensus sequence is alomst always found in genes transcribed by Pol II

A

TATA box

22
Q

does pol II requre transcription factors in order to initiate transcription

A

yes

23
Q

function of TBP portion of TFIID

A

binds to TATA box, acts as a signpost

24
Q

what base pairs are easier to pull apart

A

A-T

25
Q

what are promotor regions usually rich in

A

AT pairs

26
Q

basal transcription complex

A

compex of RNA and general transcription factors

27
Q

function of basal transcription complex

A

initiates transcription at low rate

28
Q

what do GC-rich sequences bind

A

SP-1

29
Q

what does CAAT box bind

A

NF1

30
Q

function of enchanger elements

A

proteins bind here and stimulate transcription a great deal

31
Q

what is a prerequisite to transcription

A

extensive chromatin remodeling

32
Q

toxin found in death cap mushroom

A

alpha-amanitin

33
Q

how does alpha-amnitin work

A

inhbitor of Pol II

34
Q

what happens when you inhibit pol II

A

block synthesis of mRNA

35
Q

how does rigampicin work

A

potent inhibitor of RNA polyermase

36
Q

does rifampicin affect eukaryotic pol II

A

no

37
Q

what do you use rifampicin for

A

TB