Lab - Phlebotomy & Protein-free Filtrates

Question 1

[Phlebotomy]
identify
the act of obtaining a blood sample from a vein using a needle attached to a syringe or an evacuated tube

Answer 1

phlebotomy

Question 2

[Phlebotomy]

identify the best site for venipuncture, and its specific location in the body

Answer 2

median cubital vein in antecubital fossa

Question 3

[Phlebotomy]
Which is true?
A.Venous blood is easy to get & it has most analytes in soluble form.
B.Evacuated tube method is the same as syringe method.
C.BOTA
D.NOTA

Answer 3

A

Question 4

[Phlebotomy]

What is the foremost essential factor in providing reliable lab results?

Answer 4

proper patient identification

Question 5

[Phlebotomy]
Which is true?
A. Once blood collection is done,you must allow patient to flex his/her arm.
B. A tourniquet must not be applied for more than a minute.
C. BOTA
D. NOTA

Answer 5

B

Question 6

[Phlebotomy]

How is protein removed from the blood and other biological fluids before carrying out the tests?

Answer 6

precipitated by either acids or salts of heavy metals

Question 7

[Phlebotomy]

Once protein is precipitated, what is the clear liquid called?

Answer 7

protein-free filtrate

Question 8

[Protein-Free Filtrates]
Which is false?
A. The precipitating agent is the same regardless of the analysis procedure.
B. The precipitating agent should not interfere with the substance being determined.
C. BOTA
D. NOTA

Answer 8

A

Question 9

[Protein-Free Filtrates]
The precipitating agent should provide proper \_\_ for the filtrate.
A. pressure
B. weight
C. pH
D. AOTA

Answer 9

C

Question 10

[Protein-Free Filtrates]

Enumerate 3 sources of error in PFF preparation.

Answer 10

use of unclean glassware
incorrect volumes
foaming

Question 11

[Protein-Free Filtrates]
foaming indicates 
A.inadequate sample weight
B.incomplete protein precipitation
C.too vigorous shaking
D.NOTA

Answer 11

B

Question 12

[Protein-Free Filtrates]

Enumerate 3 causes of foaming

Answer 12

improper reagent concentration
presence of an excess of anticoagulant
use of improper anticoagulant

Question 13

[Protein-Free Filtrates]
Biological fluids are precipitated with either acids or salts of heavy metals. The resulting suspension is cleared of solids by either of which 2 methods?

Answer 13

centrifugation

filtration

Question 14

[Protein-Free Filtrates]

Enumerate 3 methods of protein-free filtrate preparation.

Answer 14

Folin Wu’s Method
Somogyi Method
Trichloroacetic Method

Question 15

[Protein-Free Filtrates]

Another name for Folin Wu’s Method is ___

Answer 15

Tungstic Acid Filtrate

Question 16

[Protein-Free Filtrates]
To which method does this apply: "H2SO4 + 10% sodium tungstate is added to the sample"
A.Folin Wu's Method
B.Somogyi Method
C.Trichloroacetic Method

Answer 16

A

Question 17

[Protein-Free Filtrates]
To which method does this apply: "The resulting protein free filtrates is extremely acid, therefore its use is limited to determinations requiring very high pH"
A.Folin Wu's Method
B.Somogyi Method
C.Trichloroacetic Method

Answer 17

C

Question 18

[Protein-Free Filtrates]
To which method does this apply: "Protein is precipitated by use of zinc sulfate and barium hydroxide."
A.Folin Wu's Method
B.Somogyi Method
C.Trichloroacetic Method

Answer 18

B

Question 19

[Protein-Free Filtrates]
False about Somogyi Method
A. Advantage: non-glucose reducing agents are not present
B. There should be a balance between conc of zinc sulfate & conc of hydroxide solution
C. This method can be used in determining uric acid, creatinine and non-protein nitrogen.
D.NOTA

Answer 19

C

cannot dapat; these are left with the coagulum