Lab - Serum Glucose

Question 1

glucose + oxygen + water–> gluconic acid + hydrogen peroxide

This is catalyzed by what enzyme?

Answer 1

glucose oxidase

Question 2

From glucose oxidation, H2O2 is formed. Which enzyme catalyzes its reaction to form quinoneimine?

Answer 2

peroxidase

Question 3

From glucose oxidation, H2O2 is formed. Which two substances does it react with to form quinoneimine?

Answer 3

4-aminophenazone

phenol

Question 4

Which is not a substance present in the enzyme reagent used?
A. ammonia buffer
B. mutarotase
C. stabilizers

Answer 4

A

phosphate buffer dapat

Question 5

When opened, the enzyme reagent is stable for __ weeks when stored at 15-25 deg C.

Answer 5

2

Question 6

When opened, the enzyme reagent is stable for __ weeks when stored at 2-8 deg C.

Answer 6

6

Question 7

The serum is stable for __ hours when stored at 2-8 deg C, if serum/plasma is prepared within 30 min after collection.

Answer 7

24

Question 8

wavelength used @ spectrophotometer for the experiment

Answer 8

500 nm

Question 9

optical path @ spectrophotometer for the experiment

Answer 9

1 cm

Question 10

Which isn't a temp used @ spectrophotometer for the experiment?
A. 20 deg C
B. 25 deg C
C. 30 deg C
D. 37 deg C

Answer 10

C

Question 11

The absorbance of the standard & the sample against the reagent blank should be read within \_\_ min.
A. 15
B. 30
C. 45
D. 60

Answer 11

D

Question 12

The test is linear up to a glucose conc of __mg/dL.

Answer 12

400

Question 13

reference range of fasting blood glucose (in mg/dL)

Answer 13

70-100 mg/dL