Lab - BUN & Serum Creatinine

Question 1

Urea + water --> \_\_\_, c/o urease.
A. ammonia and carbon monoxide
B. ammonia and carbon dioxide
C. methane and nitrogen dioxide
D. NOTA

Answer 1

B

Question 2

True about Blood Urea Nitrogen determination
A. In a modified Berthelot reaction, the ammonium ions react w/ hypochlorite and salicylate to give a green dye
B. In a modified Camelot reaction, the ammonium ions react w/ hypochlorite and salicylate to give a blue dye
C. In a modified Berthelot reaction, the ammonium ions react w/ sodium chloride and salicylate to give a blue dye
D. blood serum is used as specimen here

Answer 2

A

Question 3

In BUN Det, increase of absorbance at \_\_\_ is proportional to urea concentration of sample
A. 405 nm
B. 350 nm
C. 578 nm
D. 646 nm

Answer 3

C

Question 4

Not part of reagent 1
A.sodium salicylate
B.sodium hydroxide
C.sodium nitroprusside
D.sodium hypochlorite

Answer 4

B and D = they are part of reagent 2

Question 5

True about BUN determination
A.After Reagent 2: Mix and incubate for 5 mins at 20-25 deg C OR 3 mins at 37 deg C
B.After Reagent 1: Mix, incubate for 10 mins at 20-25 deg C OR 5 mins at 37 deg C.
C.Absorbance must be read within 60 minutes

Answer 5

C

baliktad ang A and B

Question 6

Which is false?
A. All anticoagulants except EDTA can be used
B. The standard contains sodium azide (0.1%). Don’t swallow. Avoid contact w/ skin & mucous membranes
C. Reagent 2 contains sodium hydroxide and hypochlorite which are irritants.

Answer 6

A = ammonium heparin dapat

Question 7

BUN test is not influenced by
A. hemoglobin values up to 200 mg/dl 
B. bilirubin values up to 10 mg/dL
C. BOTA
D. NOTA

Answer 7

C

Question 8

in serum creatinine test: creatinine forms in alkaline solution an orange-colored complex with picric acid.
What reaction is this?

Answer 8

kinetic Jaffe reaction

Question 9

Which is not used as specimen for serum creatinine?
A. serum
B. urine
C. heparinized plasma
D. whole blood

Answer 9

D

Question 10

Wavelength at which serum creatinine test is conducted: identify

Answer 10

492 nm

Question 11

Reference range of serum BUN

Answer 11

6-20mg/dL

Question 12

reference range for serum creatinine, male

Answer 12

Male: 0.6 - 1.1 mg/dL

Question 13

reference range for serum creatinine, female

Answer 13

female: 0.5 - 0.9 mg/dL

Question 14

false about serum creatinine det
A. Reaction is not sensitive to temperature.
B. PIC is harmful when inhaled, swallowed, or in contact w/ skin.
C. Assay can be affected by presence of reducing compounds

Answer 14

A - very sensitive dapat

Question 15

Mix! After 30 sec, read abs (A1)! Read abs (A2) exactly after 2 mins!
In what test is this applicable?

Answer 15

serum creatinine