Lab - Bilirubin, AST, ALT

Question 1

Bilirubin reacts with diazotized sulphanilic acid (DSA) to form a diazo dye. What color?
A. red
B. blue
C. green

Answer 1

A

Question 2

Which is false?
A. Water-soluble bilirubin glucoronides react directly with diazotized sulphanilic acid
B. Sulphanilic acid & sodium nitrite form diazotized sulphanilic acid
C .Bilirubin + DSA —> Total Azobilirubin

Answer 2

(C - Direct kasi dapat! pag may accelerator, total na.)

Question 3

[Serum Bilirubin]
Absorbance of the diazo dye at \_\_\_is directly proportional to bilirubin concentration in sample
A. 345 nm
B. 546 nm
C. 467 nm

Answer 3

B

Question 4

Differentiate the procedure of measuring total bilirubin fr measuring direct bilirubin.

Answer 4

time of incubation! direct bil - exactly 5 min. total bil - 10 to 30 min

Question 5

A.Reference range of total bilirubin in adults(mg/dL): ___

B.Reference range of direct bilirubin in adults(mg/dL): __

Answer 5

A. 1.1 mg/dL

B. 0.25 mg/dL

Question 6

Which is false?
A. It is important to ensure the bilirubin reagent and the nitrite reagent are thoroughly mixed before adding the sample.
B. Bilirubin levels may be increased if the samples are exposed to light.
C. Hemolysis will lower the bilirubin value due to the inhibitory effect of hemoglobin on the diazo reaction.

Answer 6

B - decreased dapat

Question 7

[ALT]
Alanine aminotransferase catalyzes the reaction between 2-oxoglutarate and L-alanine. Specify the products that are formed

Answer 7

L-glutamate and pyruvate

Question 8

[ALT]
Pyruvate reacts with \_\_ and hydrogen ion to form \_\_ & lactate
A. FADH2 --> FADH
B. FADH --> FADH2
C. NADH --> NAD+
D. NAD+ --> NADH

Answer 8

C

Question 9

[ALT]
ALT conc is measured spectrophotometrically. It is measured in any of the ff wavelengths except:
A. 365 nm
B. 340 nm
C. 334 nm
D. 321 nm

Answer 9

D

Question 10

[ALT]
False about reference range  of ALT:
A. Men have bigger amount than women
B. Women have bigger amount than men
C. It depends on the temperature

Answer 10

A

Question 11

[ALT]

specimen for testing

Answer 11

blood serum

Question 12

[AST]
Aspartate aminotransferase catalyzes a reaction that yields L-glutamate & oxaloacetate.
What are the reactants?

Answer 12

2-oxoglutarate + L-aspartate

Question 13

[AST]

When oxaloacetate reacts with NADH and Hydrogen ion, what is formed aside from NAD+?

Answer 13

L-malate

Question 14

[ALT/AST]

difference of TRIS buffers?

Answer 14

pH! for ALT: pH 7.5 ; for AST: pH 7.8

Question 15

[AST]
False about AST reference range
A. women have higher reference value range than men
B. men have higher reference value range than women
C. it depends on the temperature

Answer 15

B

Question 16

[AST]
False about AST reference range
A. normally, in terms of amt: ALT > AST
B. normally, in terms of amt: AST > ALT
C. it depends on the temperature

Answer 16

A