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PH 166 > Spectrophotometry and Wavelength Selection > Flashcards

Spectrophotometry and Wavelength Selection Flashcards

1
Q

[Spectrophotometry]

Differentiate photometer from spectrophotometer

A

Photometer - measure light w/o considering wavelength

Spectrophotometer - measure light intensity in a narrower wavelength

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2
Q

[Spectrophotometry]

Spectrophotometers use ____ to isolate a narrower range of wavelength

A

monochromator systems (diffraction grating or prism)

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3
Q

[Spectrophotometry]
___ should be the one where the maximum absorption takes place when the specimen being analyzed is compared to the reagent blank

A

Wavelength

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4
Q

[Spectrophotometry]
Identify
obtain the wavelength where maximum absorption occurs for serum protein based on the biuret reaction

A

Serum-protein concentration-absorbance curve

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5
Q

[Spectrophotometry]

Energy that is absorbed or transmitted in analytical methodologies in clinical chemistry

A

Radiant energy

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6
Q

[Serum Cholesterol]

First reaction in detection of serum cholesterol is enzymatic hydrolysis. What is hydrolyzed?

A

cholesterol ester

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7
Q

[Serum Cholesterol]

First reaction in detection of serum cholesterol is enzymatic hydrolysis. What two substances are formed?

A

cholesterol

fatty acid

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8
Q 
[Serum Cholesterol]
Catalyzes first reaction in cholesterol det
A.cholesterol esterase 
B.cholesterol oxidase
C.cholesterol fumarase
D.NOTA
A

A

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9
Q 
[Serum Cholesterol]
Catalyzes second reaction in cholesterol det
A.cholesterol esterase 
B.cholesterol oxidase
C.cholesterol fumarase
D.NOTA
A

B

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10
Q 
[Serum Cholesterol]
What is formed after the oxidation of cholesterol?
A.quinoneimine
B.4-aminoantipyrine
C.cholestene-3-one
D.NOTA
A

C

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11
Q 
[Serum Cholesterol]
Which isn't part of the enzyme reagent used in the experiment?
A. phosphate buffer
B. phenol
C. cholestene-3-one
D. sodium azide
A

C

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12
Q 
[Serum Cholesterol]
Which is not the same for serum glucose & serum cholesterol tests?
A. indicator formed
B. reference ranges
C. reagent stability
D. spectrophotometric details
A

B

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13
Q

[Serum Cholesterol]

wavelength used for the experiment

A

500 nm

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14
Q

[Serum Cholesterol]

reference range of borderline high for cholesterol

A

200-239 mg/dL

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15
Q

[Serum Cholesterol]
Only serum cholesterol value is needed for completely evaluating blood cholesterol.

T/F

A

F

complete lipid profile dapat

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16
Q 
[Serum Cholesterol]
Lipid profile teting should be performed in all adults 20 years and older, and should be repeated at least once every \_\_ years.
A. 5
B. 10
C. 15
D. 20
A

A

PH 166 (28 decks)

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