BIOLOGY/BIOCHEM QUESTIONS Flashcards
1
Q
Name two possible sources of error when estimating Km and Vmax from a Lineweaver-Burk plot.
A
- The inability to obtain a negative value of 1/[S] often requires extrapolation of the linear fit over a long stretch lacking data points in order to determine the x-intercept
- Errors in the data obtained at low substrate concentration have a disproportionate impact on the determination of the best linear fit.
2
Q
How is the Km and Vmax affected by competitive inhibition?
A
Km decreases. Vmax stays the same.
3
Q
How is the Km and Vmax affected by noncompetitive inhibition?
A
Km stays the same. Vmax decreases.
4
Q
How is the Km and Vmax affected by uncompetitive inhibition?
A
Km decreases. Vmax decreases.
5
Q
How is the Km and Vmax affected by mixed inhibition?
A
Km varies. Vmax decreases.
6
Q
T/F: Prosthetic groups are non-protein organic molecules associated with enzymes?
A
True
7
Q
T/F: Prosthetic groups are added to proteins after translation?
A
True
8
Q
T/F: Prosthetic groups are needed in order for some enzymes to catalyze reaction?
A
True
