Studying Cells And Tissues W2 Flashcards

1
Q

Under normal EM conditions what do you expect to find in the nucleus (6)

A
Nuclear membrane 
Nuclear pores 
Nucleolus
Heterochromatin 
Euchromatin 
Chromosomes
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2
Q

Under EM what colour is euchromatin

A

Light grey

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3
Q

Under EM what colour is heterochromatin

A

Dark grey

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4
Q

Why do we have nuclear pores

A

To allow mRNA to leave the nucleus

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5
Q

What is the space inside the endoplasmic reticulum called

A

Cistern

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6
Q

Where do metabolic processes occur with reference to the endoplasmic reticulum

A

On the surface and within the cistern

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7
Q

Give examples of metabolic processes that occur within/on surface of endoplasmic reticulum (4)

A

Protein synthesis and modification

Lipid and steroid synthesis

Detoxification

Manufacture of cellular membranes

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8
Q

Where are ribosomes manufactures

A

In the nucleolus

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9
Q

Do ribosomes have to be attached to the ER

A

No

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10
Q

What are ribosomes (and therefore rER) composed of

A

Ribonucleic acid

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11
Q

If ribosomes (and therefore rER) composed of ribonucleic acid what stain do they attract

A

Basic stain - dark purple

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12
Q

What colour does protein stain under a LM

A

Pink

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13
Q

What do cells that produce protein for export into a duct look like (2regions)

A

Have a basophilic region where most of the rER is and then acidophilus regions on the inside where the secretory vesicles with the protein are

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14
Q

Ribosomes attached to the endoplasmic reticulum are involved in ……… ……..

A

Protein synthesis

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15
Q

How is the ER involved in protein synthesis ?

A

At the rER proteins are packaged I nto vesicles for export

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16
Q

Where can protein synthesis occur

A

rER

Cytosol

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17
Q

What can the rER do to accomodate for accumulating proteins

A

It expands

18
Q

Smooth ER is primarily found in cells involved in….(3)…production

A

Steroid

Cholesterol

Triglyceride

19
Q

Smooth ER is also found in cells that function to …….. ……. …….eg (2)

A

Detoxify toxic material

Eg alcohol and barbiturates

20
Q

Examples of cells that would have smooth ER present (1) that are present within (3) organs

A

Hepatocytes

Present in
Testis
Ovaries
Adrenal gland

21
Q

The Golgi complex is is an area of (2)

Of substances manufactured by the (2)

A

Packaging and modification

Smooth ER
Rough ER

22
Q

Golgi body has two faces what are the names

A

Cis and trams face

23
Q

Name of Golgi complex face, facing ER

A

Cis

24
Q

Name of Golgi complex face, facing away from ER

A

Trans face

25
Q

Which face of Golgi complex receives becomes from ER

A

Cis

26
Q

Which face of Golgi complex releases vesicles

A

Trans

27
Q

What happens to vesicles that come out of trans face of Golgi complex

A

Either go to membrane

Or out of the cell

28
Q

Anything manufactured on the rER goes where

A

Outside of the cell or to cell membrane

Things that stay in the cell are made by free ribosomes floating around in the cell

29
Q

Are lysosomes acidic or basic

A

Acidic

30
Q

What enzymes do lysosomes contain

A

Hydrolytic

31
Q

What are perixisomes

A

Self replacing organelles that contain oxidative enzymes

32
Q

What do peroxisomes do

A

Involved in catabolism of long fatty acid chains

33
Q

Average lifespan of mitochondria

A

10 Days

34
Q

What are cristae

A

I foldings of inner mitochondrial membrane

35
Q

whats the name of the dots seen inside mitochondria

A

matrix granules

36
Q

2 types of cristae (ie their look)

A

shelf like cristae

tubular cristae (under em look like small circles)

37
Q

mitochondria with tubular cristae are most often found in mitochondria in cells that produce……

A

steroids

38
Q

mitochondria with shelf like cristae are found in mitochondria in cells that produce …..

A

protein

39
Q

Example of organs which contain cells that may have mitochondria with tubular cristae (3)

A

testes ovaries and adrenal glands

40
Q

can the cytoskeleton be seen using a LM

A

not unless special staining is used

41
Q

much of the general eosenophilia of the cytoplasm of a cell may be due to the …..

A

cytoskelton

42
Q

what does the cytoskeleton look like under an EM

A

cant really see it, but the grainy nature of cytosol is probably elements of the sytoskeleton