ames test quiz

Question 1

What is the ames test

Answer 1

whether a chemical can mutate DNA

Question 2

Mutagen

Answer 2

a chemical substance or physical agent that can induce changes in the base sequence of DNA

Question 3

Carcinogen

Answer 3

mutation that can cause cancer

Question 4

Types of base mutations

Answer 4

silent, nonsense, missense

Question 5

silent

Answer 5

change in a base that will still code for the same amino acid

Question 6

nonsense

Answer 6

codon will code for the sequence to stop

Question 7

missense

Answer 7

code for another amino acid

Question 8

frameshift mutation

Answer 8

nucleotides are inserted or deleted, shifting the sequence

Question 9

what type of mutation did the M starin have

Answer 9

a missense mutation in the histidine gene

Question 10

mutation that f strain has

Answer 10

deletion frameshift mutation

Question 11

for a mutation to occur on the media without hist, the bacteria needs to have

Answer 11

back mutation

Question 12

what is a back mutation

Answer 12

mutated genes that will revert back to their wild type if a second mutation occurs.

Question 13

explain the experiment

Answer 13

m strain with missense mutation and f stain with frameshift mutation. both mutations inhibit the bacteria to grow. the strains lack the sequence to reproduce. these strains are placed mm plates with either NaAz(m strain) or 4-nitro ( f strain). The plates do not contain histidine. If colonies appear, these two chemicals cause a back mutation. The bacteria can now reproduce and colonies would appear. That means it tested positive, and the chemical can now be further tested to see if it’s a possible carcinogen.

Question 14

sodium azide (NaAz)

Answer 14

is known to cause base mutations

Question 15

4nitro

Answer 15

known t cause frameshift mutations

Question 16

toxicity test

Answer 16

est chemicals that are toxic to the bacteria.
the chemical is toxic if there is a circle of death or no bacteria growth