Automation in Histopathology (F) Flashcards
What are the advantages of the manual method?
1) Very easy to set up. No big capital investment
2) Flexibility
3) Helpful in the training of MT interns
4) Few rgnt needed if small sx
What are used in manual method?
Jars
What are the disadvantages of the manual method?
1) MT in charge sometimes forgets the time
2) Agitation is not consistent
3) Failure to drain the basket properly before transferring to next station
4) Longer time to release the slides
What are the do’s and don’ts w/ the manual method?
1) Use cassettes and baskets
2) Better to process at daytime
3) Use big containers, not small jars
What are the advantages of automated tissue processing?
1) Leave alone
2) Continuous agitation
3) Can program when to process
What are the do’s and don’ts of automated tissue processing?
1) Be sure to connect to alternative power supply (generator)
2) Have more than 1 staff (preferably all) know how to operate the machine properly
3) Preventive maintenance
What are the advantages of (/ using) tissue embedding machine?
1) Very orderly operation
2) W/ hot plate and cold plate
3) Easy dispensing of paraffin
What are the disadvantages of automation?
1) Capital investment
2) Brownout
3) Machine malfxn
4) Some med staff does not know hot to operate the machine
5) Machine maintenance
What is the tissue processing schedule (w/ tissue processor)?
1) Station 1: 10% neutral buffered formalin (NBF) - 1 hr
2) Station 2: 10% NBF - 1 hr
3) Station 3: 50% ethanol - 1 hr
4) Station 4: 70% ethanol - 1 hr
5) Station 5: 80% ethanol - 1 hr
6) Station 6: absolute alcohol - 1 hr
7) Station 7: acetone - 1 hr
8) Station 8: xylene - 1 hr
9) Station 9: xylene - 1 hr
10) Station 10: paraffin wax - 1 hr
11) Station 11: paraffin wax - 1 hr
12) Station 12: paraffin wax - 1 hr
What is the total of hrs for tissue processing schedule (w/ tissue processor)?
12 hrs
What is the manual processing schedule?
1) Station 1: 10% formalin - 3 hrs
2) Station 2: 10% formalin - 1 hr
3) Station 3: 70% alcohol - 1 hr
4) Station 4: 80% alcohol - 1 hr
5) Station 5: 90% alcohol - 1 hr
6) Station 6: absolute alcohol - 1 hr
7) Station 7: absolute alcohol - 2 hrs
8) Station 8: chloroform - 2 hrs
9) Station 9: paraffin wax - 80 mins
What is the total # of hrs for manual processing schedule?
13 hrs 20 mins
What are the advantages of microwave technology?
1) Same day dx
- > from overnight processing to same-day processing
2) Less # of rgnts
- > from 12 - 14 rgnts to 2 - 3 rgnts only
3) Low toxicity of rgnts
- > from the use of hazardous rgnts (such as xylene - formalin) to the use of low toxicity rgnts (such as FineFix, ethanol, isopropanol, JFC, and ProWave)
4) Can modify timing easily
- > depending on the thickness of tissue / type of tissue being cut
Answer the ff questions w/ regards to the given thickness of tissue (in relation to the principle of rapidity of processing time w/c is related to the 1 of the advantage of microwave technology w/c is can modify timing easily):
1) What is the time duration of the given tissue (w/out fix)?
2) What is the time duration of the given tissue (w/ fix)?
Given thickness of tissue: 1.5 mm
1) 30 mins
2) 40 mins
Answer the ff questions w/ regards to the given thickness of tissue (in relation to the principle of rapidity of processing time w/c is related to the 1 of the advantage of microwave technology w/c is can modify timing easily):
1) What is the time duration of the given tissue (w/out fix)?
2) What is the time duration of the given tissue (w/ fix)?
Given thickness of tissue: 3 mm
1) 1.20 hr
2) 1.45 hr
