B cell activation and antibody production part 2

Question 0

Progeny of the clone may differentiate into plasma cells that produce___ or isotype switch

Answer 0

IgM…

Question 1

activation of B cells is initiated by?

Answer 1

specific antigens by surface BCR’s

Question 2

B cell differentiation results in an increasing fraction of the ___ protein produced as the secreted form

Answer 2

U (meu)

Question 3

in T dependent response, some the progeny activated IgM and IgD expressing B cells undergo ? and produce abs with heavy chains of different classes. delta, alpha, and epsilon

Answer 3

Heavy Chain Isotype switching

Question 4

Where does Isotype switching occur?

Answer 4

germinal centers driven by Follicular T helper cells

Question 5

class switching occurs in response to?

Answer 5

changing the constant regions of heavy chains

Question 6

Activated B cells can secrete IFN-gamma and isotype switch to a plasma cell that secretes?

Answer 6

IgG to activate complement system.

Question 7

helminth infections likely influence Follicular T helper cell differentiation to these cells, which produce Th-2 type cytokines during the germinal center reaction.

Answer 7

IL-4+ Follicular T helper cells

Question 8

B cells in mucosal tissue switch to?

Answer 8

IgA secreting plasma cells

Question 9

Switching to IgA involves?

Answer 9

TGF-beta

Question 10

in Immunodeficiencies related to the CD40-CD40L axis the antibody response to protein antigens is dominated by

Answer 10

IgM antibodies

Question 11

The mature B cells first produce IgM Ig’s. the rearranged VDJ sequences are positioned directly adjacent to other C genes. Every C gene is preceded by ___that controls the rearrangement process

Answer 11

Switching the S sequence

Question 12

what is the key enzyme required for isotype switching and affinity maturation?

Answer 12

Activation Induced Cytosine Deaminase (AID)

Question 13

AID expression is activated by?

Answer 13

CD40 signals from follicular T cells

Question 14

AID deaminates cytosines in ss-DNA converting Cytosine residues to?

Answer 14

Uracil residues

Question 15

During the actual binding of B cells to the T helper cell surface, which is not involved?

Answer 15

Ig

Question 16

if you could analyze at the molecular level a plasma cell making IgA ab, you wouldnt find?

Answer 16

mRNA specific for U (meu) heavy chains

Question 17

Affinity maturation is observed ONLY in antibody response to?

Answer 17

T-dependent protein antigens

Question 18

somatic hypermutation of Ig V genes and selection of B cells with high affinity Ag recep

Answer 18

…..

Question 19

what happens in the dark zone of germinal centers?

Answer 19

proliferation and point mutation in V-region of H/L chains

Question 20

Mutated Ig with higher affinity binds antigen presenting FDC and survives in the?

Answer 20

light zone of germinal centers

Question 21

mutations in which genes, increase with time after immunization and with repeated injections?

Answer 21

V genes

Question 22

Multiple myeloma is a cancer which has arisen from a

Answer 22

plasma cell

Question 23

many non protein antigens, such has polysaccharides and lipids, stimulate ab production in the absence of Th cells

Answer 23

…..

Question 24

T-independent responses involve which type of antigens?

Answer 24

non-protein: polysaccharides and lipids

Question 25

T-dependent responses involve which type of antigens?

Answer 25

protein

Question 26

these cells respond readily to the T-independent antigens mainly in the peritoneum and in mucosal sites

Answer 26

B-1 cells

Question 27

CD4+ helper T cells cannot recognize which type of Antigens?

Answer 27

T-independent antigens: polysaccharides, glycolipids, and nucleic acids

Question 28

what is the function of the complementarily determining region of an Ig?

Answer 28

determine antibody specificity and are found on both the heavy and the light chains

Question 29

if you wanted to check for the presence of memory B cells from your vaccine subjects by taking some of their B cells and re-stimulating them in vitro with antigen, the best place to get some memory cells would be from?

Answer 29

Blood