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RUSVM Bacteriology Gram Positives > Bacillus > Flashcards

Flashcards in Bacillus Deck (27)
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1

Bacillus descriptive statement

LARGE Gram + rods -*Motile*(most) - Catalase +, Oxidase -, Aerobes/facultative anaerobes- Endospores (persist in environment 50+ yrs)

2

Bacillus anthracis Transmission

Ingestion/inhalation/ invasion of wounds by spores--> complex toxins

3

Bacillus anthracis Virulence Factors

*Capsule w/ poly D glutamic acid* - Protective antigen - Edema factor (EdTx or EF) - Lethal Factor (LeTx or LF)

4

Bacillus anthracis Edema factor effects

Adenylate cyclase upsets water homeostasis

5

Bacillus anthracis Protective antigen

Binds EF and LF - Essential for those factors to function - (PA+LF=Lethal Activity) (EF+PA=edema) (EF+LF=inactive) (PA+LF+EF=edema & necrosis, lethal)

6

Bacillus anthracis Lethal factor

Zinc metalloprotease, essential for lethal effects

7

Bacillus anthracis aka...

Anthrax!! Category A bioterrorism agent!

8

Bacillus anthracis colonies

"Ground Glass" - "Medusa Head colony"

9

When I say medusa head colony, you say...

B. anthracis!

10

Bacillus anthracis susceptibility

Ruminants > Carnivores > birds=resistant

11

What should you do if you find a big, dead, bloated cow with no signs of rigor mortis?

NOT NECROPSY! Report it, get authorities to do it properly or on-site incineration

12

Bacillus anthracis: Ruminants (including post mortem description)

Septicemia, rapidly fatal. Epistaxis - Postmortem: rapid bloating, incomplete rigor, widespread hemorrhages, edema, large soft spleen

13

Bacillus anthracis in Pigs

Less fatal (similar in carnivores) - Edema of throat & head - Mesenteric & regional lymphadenitis - Dysentary (Swollen head, bloody diarrhea)

14

Bacillus anthracis in horses

SQ edema of thorax, abdomen, legs - Swelling of pharynx, asphyxiation - In septicemic cases, extensive ecchymoses and splenomegaly (Swollen trunk / pharynx / legs)

15

If I say edematous swelling of throat & head of a pig or carnivore, you say...

Bacillus anthracis!

16

If I say SubQ edema of thorax, abdomen or legs of a horse, you might say....

Bacillus anthracis!

17

If I say multifocal, hemorrhagic enteric lesions, you say...

Dysentery!

18

If I say extensive ecchymoses and splenomegaly in a horse, you might say

Bacillus anthracis - septicemic case!

19

Bacillus anthracis in humans

Cutaneous anthrax (most common) - Inhalation anthrax ("Wool sorters' disease) - GI anthrax

20

If I say Wool Sorters' disease, you say....

Bacillus anthracis in humans!

21

What does Bacillus lichemiformis cause, in what species?

Sporadic abortion in cattle & sheep

22

Bacillus cereus

"Be serious" - Human food poisoning - Rare mastitis in cows

23

Bacillus larvae

American foulbrood in bees

24

What does a HIGH dose of LeTx or LF cause?

Apoptosis of macrophages

25

What does a LOW dose of LeTx or LF cause?

Cytokine "storm" --> multiorgan dysfunction - endotoxemia

26

Bacillus antracis pathogenesis

Spores in environment --> Macrophages phago. --> AtxA or AcpA trigger "activation" of bacteria --> release LeTx and EdTx, capsule, other toxins --> Escape --> hyperproduction of cytokines --> gelatinous edema & bleeding from EVERYWHERE

27

Bacillus anthracis Post-mortem

Widespread hemorrhages - Black,engorged, friable spleen - Tarry, non-clotting blood - Absence of rigor