Bacterial Genetics Flashcards Preview

D1S U1 Microbiology > Bacterial Genetics > Flashcards

Flashcards in Bacterial Genetics Deck (17):
1

The rate of DNA replication of E. coli is determined by rate of ______, not growth rate

Initiation

2

Nucleotide is changed

Base substitution

3

Nucleotide change results in stop codon

Nonsense

4

Nucleotide change results in different amino acid

Missense mutation

5

Transfer of plasmid DNA through sex pili

Conjugation

6

Bacteria phage (virus) accidentally inject bacterial DNA into host bacterial cell

Transduction

7

DNA that can insert or excise itself from the chromosome or plasmid

Transposable elements

8

Ability to take up DNA from environment, mediated by quorum sensing

Natural Transformation

9

Bacteria sense their environment through _______, allowing them to control which proteins are made and how much

Quorum Sensing

10

TCS (Two Component Regulatory System)- regulates genes in response to an ______ signal

Environmental

11

RNA polymerase binds to ____ located upstream of gene start codon

Promoter

12

Random mutations are introduced creating a mutant library that can be screened

Random Mutagenesis

13

A gene is replaced by an antibiotic marker

Allelic Replacement

14

A gene from a pathogenic organism is expressed in a non-pathogenic strain to see if the strain becomes virulent (I.e. Host cell invasion)

Complementation

15

Ability to manipulate genes- make mutations or knockout genes

Functional Genomics

16

Uses a gene chip (has a fragment of each gene of a bacteria), hybridizes two populations of differently labeled RNA samples to determine the differences in level of expression

Microarray

17

Total RNA is isolated and sequenced using Illuminia technology. RNA reads are counted to determine differential gene expression. (More modern than Microarray)

RNA sequencing