Bacteriology

Question 1

Difference in cell wall between gram + and Gram - bacteria?

Answer 1

Gram - has LPS present and thinner peptidoglycan (periplasmic space present)

Question 2

Techoic acid found on gram negative or positive bacteria?

Answer 2

Gram +

Question 3

What causes Lipid A septic shock?

Answer 3

LPS on outer membrane of gram -

Question 4

What do obligate anerobes lack?

Answer 4

superoxide dismutase and catalase

Question 5

inhibits transformation?

Answer 5

DNAse

Question 6

Viral filter inhibits?

Answer 6

Transduction

Question 7

Conjugation inhibited by?

Answer 7

bacterial filter

Question 8

Which genetic excahnge is with environmental DNA?

Answer 8

Transformation

Question 9

Which gram + coccie is catalase positive and which is negative?

Answer 9

Catalase positive is staph and negative is strep

Question 10

Staph aureus is ccoagulase + or negative?

Answer 10

+

Question 11

Staph aureus responsible for what?

Answer 11

TSS
Scalded skin syndrome
Wound infection
honey colored crusts

Question 12

Coagulase - and novobiocin sensitive?

Answer 12

staph epidermis

Question 13

Staph epidermis causes what?

Answer 13

think catheters/implanted devices due to biofilm

Question 14

Staph sapro is novobiocin resistant of sensitive?

Answer 14

resistant

Question 15

What does staph sapro cause?

Answer 15

UTI

Question 16

Catalase negative (So we know its strep) beta hemolytic and bacitracin sensitive?

Answer 16

S. Pyogenes

Question 17

S. pyogenes (Group A strep) symptoms?

Answer 17

pharyngitis, scarlet fever, gomerulo nephritis

Question 18

S. agalactiae is bacitracin resistant or sensitive?

Answer 18

resistant

Question 19

Neonatal sepsis and meningitis caused by what kind of strep?

Answer 19

agalactiae

Question 20

Catalase negative alpha hemolytic and optochin sensitive is…

Answer 20

S. pneumo

Question 21

What differences between S. pneumo and S. mutans (viridans)?

Answer 21

S. pneumo has capsule and is bile soluble

Question 22

Dental caries?

Answer 22

S. viridans

Question 23

bacteria capsule purpose?

Answer 23

to prevent it from being phagocytosed

Question 24

Anterior nares?

Answer 24

Staph aureus

Question 25

Superantigen?

Answer 25

Staph aureus and strep pyogenes

Question 26

Which type of gram + cocci causes food poisoning?

Answer 26

S aureus

Question 27

M protein

Answer 27

Strep pyogenes

Question 28

Rheumatic fever?

Answer 28

Strep pyogenes (group A strep)

Question 29

Enterobacteriae different from other gram negative bacilli because they are all (5)

Answer 29

``` facultative anerobic ferment glucose reduce nitrate to nitrite oxidase (-) all have LPS or endotoxin on outer membrane ```

Question 30

How to tell difference between enterobacteriae?

Answer 30

Look at sugar fermentation pathway

Question 31

What produces tryptophanase and what does it do?

Answer 31

E. Coli and it cleaves tryptophan, producing indole

Question 32

ETEC is known as....

Answer 32

Traveler's diarrhea

Question 33

EHEC main symptom?

Answer 33

bloody diarrhea

Question 34

common type of EHEC?

Answer 34

O157: H7

Question 35

How do screen for EHEC?

Answer 35

Sorbitol MacConkey agar and confirm with shiga toxin assay

Question 36

Hemolytic uremic syndrome (think hamburger!) seen in...

Answer 36

EHEC

Question 37

Shiga toxin?

Answer 37

EHEC

Question 38

Non-motile gram - bacilli?

Answer 38

Shigella

Question 39

Shigella and lactose?

Answer 39

Does not ferment

Question 40

Shigella transmission?

Answer 40

fecal-oral

Question 41

Salmonella vs. Shigella?

Answer 41

Salmonella is motile but also non-fermenting of lactose

Question 42

Repiles?

Answer 42

Salmonella

Question 43

Salmonella produces

Answer 43

H2S

Question 44

Raw eggs ingestion?

Answer 44

Typhimurium (Salmonella)...usually connected to sanitary conditions

Question 45

Rose spots

Answer 45

Typhi (salmonella)

Question 46

How to acquire Typhi

Answer 46

ingestion of fecally contaminated water

Question 47

Morbidity of Typhi due to?

Answer 47

enteric fever

Question 48

Bubonic plague?

Answer 48

Yersinia pestis

Question 49

Lymphadenopathy in groin, black skin lesion?

Answer 49

Yersinia pestis

Question 50

Cold temps, mimics appendicitis, puppy feces?

Answer 50

Yersinia enterocolitica

Question 51

B burgdorferi transmission?

Answer 51

ticks

Question 52

R. Rickettsii transmission?

Answer 52

ticks

Question 53

Giemsa stain: safety pin

Answer 53

Yersinia

Question 54

currant jelly URI

Answer 54

Klebsiella

Question 55

FAST lactose fermenters (gram negative bacilli)

Answer 55

Klebsiella, E. Coli, Enterbacter

Question 56

Gram posiive bacilli non spore forming anaerobic

Answer 56

Actinomyces, Lactobacillus

Question 57

Gram posiive bacilli non spore forming aerobic

Answer 57

Cornyebacterium diptheriae, listeria, tropheryma whipplei

Question 58

Grey pseudomembrane

Answer 58

Corynebacterium diptheriae

Question 59

Whipples disease, PAS positive, poor gram staining

Answer 59

Tropheryma whipplei

Question 60

Chinese letters

Answer 60

Corynebacterium diptheriae

Question 61

Bullneck

Answer 61

Cornyebacterium dip