Biological Resources Flashcards

1
Q

Lactobacillus in yoghurt making

A
  1. Equipment sterilised to kill any unwanted bacteria
  2. Milk is pasteurised (heated) at 85 degrees Celsius to kill any natural bacteria the milk contains
  3. Milk is homogenised - dispersed fat into globules (makes smaller)
  4. Milk is cooled to 30-40 degrees to stop bacteria being killed. It is mixed with lactobacillus - this digests milk proteins and ferments lactose, producing lactic acid
  5. Milk is incubated at this temperature
  6. During fermentation, the proteins in the milk denature - not the bacteria due to a drop in pH - this results in the mixture thickening. Thickened yoghurt is stirred and cooled to 5 degrees. This halts the action of bacteria
  7. Flavourings, colourings and fruit can be added before packaging
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What affects crop yield

A

the rate of photosynthesis, which is affected by water, light, carbon dioxide and temperature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

method to create ideal conditions for photosynthesis

A

using a greenhouse

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how do greenhouses help create ideal conditions for photosynthesis

A
  • keeping plants enclosed makes it easier to keep them free from pests and disease
  • helps farmers control water supplied to crops
  • commercial farmers often supply artificial light even after the sun goes down to give plants more time to photosynthesis
  • greenhouses trap the suns heat to keep plants warm
  • in winter, a heater can be used to keep temperature at an ideal level
  • can increase amount of CO2 available (e.g. paraffin heater to heat the place which released CO2 as a by product)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what is used on plants to ensure crops have enough minerals

A

fertilisers:
plants need certain minerals (e.g. nitrogen, potassium and phosphorus) to make proteins to allow them to grow
sometimes there are not enough minerals available, so farmers use fertilisers to provide more and increase crop yield

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

how does pest control stop pests eating crops

A
  1. pests include microorganisms, insects and mammals
  2. pests that feed on crops are killed using various methods of pest control
  3. this means that less plants are damaged or destroyed, increasing crop yield
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what are pesticides

A

form of chemical pest control
often poisonous to humans, so must be used carefully to keep amount of pesticide in food below a safe level
some pesticides also harm wildlife

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

biological control

A

alternative to using pesticides
means using other organisms to reduce the number of pests, either by encouraging wild organisms or adding new ones
the helpful organisms could be predators, parasites or disease-causing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

pesticides vs biological control

A
  • biological control can often have a longer lasing effect than pesticides and be less harmful to wildlife
  • however introducing new organisms can cause problems (e.g. cane toads were introduced to Australia to eat beetles, but they are now a major pest themselves as they poison native species that eat them)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

what do fermenters do

A

can provide optimum conditions for microorganisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

how do fermenters work - pH monitoring

A

pH monitored and kept at optimum level for enzymes to work efficiently
this keeps the rate of reaction and product yield as high as possible

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

how do fermenters work - nutrients

A

nutrients needed by the microorganisms for growth are provided in the liquid culture medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

how do fermenters work - paddles

A

microorganisms are kept in contact with fresh medium by paddles that circulate the medium around the vessel
this increases product yield as the microorganisms can always access the nutrients needed for growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

how do fermenters work - temperature

A

temperature is monitored and kept at an optimum level
a water-cooled jacket makes sure it doesn’t get too hot so that the enzymes do not denature

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

how do fermenters work - sterilised

A

vessels are sterilised between uses with superheated steam to kill unwanted microbes
having aseptic conditions increases the product yield because the microorganisms aren’t competing
it also means that the product doesn’t get contaminated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

how do fermenters work - oxygen

A

if the microorganisms need oxygen for respiration, its added by pumping in sterile air
this increases the product yield because the microorganisms can always respire to provide the energy for growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

key features of fermenters

A

water-cooled jacket
paddles
superheated steam
pumped sterile air
culture medium

18
Q

yeast in breadmaking

A
  1. initially, yeast will respire aerobically, producing carbon dioxide and water
  2. when air runs out, conditions become anaerobic, releasing carbon dioxide and ethanol - known as fermentation
  3. the yeast produces enzymes that break down the starch, releasing sugars that can be used for anaerobic resp.
  4. the carbon dioxide is trapped in bubbles in the dough, as the pockets of gas expand, the dough begins to rise
  5. the dough is then baked in an oven where the yeast continues to ferment until the temperature of the dough rises enough to kill the yeast
  6. any ethanol produced is evaporated away
  7. the yeast dies meaning the bread stops rising, but pockets are left in the break where the CO2 was trapped
19
Q

selective breeding

A

the process by which humans breed plants/animals with desired characteristics
they want to obtain bigger food yields from crops and stock animals to increase food supply

20
Q

process of selective breeding

A
  1. humans select desired characteristics
  2. cross breeding of male and female with desired characteristics
  3. selection of offspring showing desired characteristics
  4. repeat the process over many generations until allele for desired characteristic increases in frequency
21
Q

fish farming key features

A
  1. fish are kept in cages in the sea - less energy from swimming used
  2. cage also protects them from interspecific predation
  3. fed a diet of pellets that are controlled to maximise the energy they get so the fish grow more
  4. young fish are put in special tanks to survive
  5. younger fish are separated from older fish to prevent intraspecific predation
  6. fish in cages are more prone to diseases and parasites - biological pest control can be used
  7. fish can be selectively bred to produce faster growing, less aggressive fish
22
Q

what are fish farms designed for

A

to produce as many fish as possible

23
Q

fish being farmed in tanks

A

conditions can be controlled:
- water monitored to ensure temp, pH and O2 level is ok
- easy to control the amount and type of food supply
- water can be removed and filtered to get rid of waste food and faeces - keeps water clean for fish and avoids pollution

24
Q

what can enzymes do to DNA

A

cut up DNA or join DNA pieces together

25
restriction enzymes
recognise specific sequences of DNA and cut the DNA at these points
26
ligase enzymes
used to join 2 pieces of DNA together
27
what is recombinant DNA
Two different bits of DNA stuck together
28
what are plasmids
small, circular molecules of DNA that can be transferred between bacteria
29
what is a vector
something that is used to transfer DNA into a cell
30
two types of vector
plasmids viruses
31
what are viruses
insert DNA into the organisms that they infect
32
process of genetic engineering
1. DNA you want to insert is cut out with a restriction enzyme 2. the vector DNA is then cut open using the SAME restriction enzyme 3. the vector DNA and DNA you are inserting are mixed together with ligase enzymes 4. the ligases join the two pieces of DNA together, to produce recombinant DNA 5. the recombinant DNA is inserted into other cells, e.g. bacteria 6. these cells now use the gene you inserted to make the protein you want (cell that contains the gene is now transgenic - meaning it contains genes transferred from another species)
33
transgenic meaning
contains genes transferred from another species
34
how can genetically modified plants increase food production
resistance to insects/herbicides
35
how does making crops insect resistant help
this means that farmers don't have to spray as many pesticides so wildlife that eats the crop isn't harmed also increases crop yield
36
how does making crops herbicide resistant help
farmers can spray their crops to kill weeds without effecting the crop itself also increases crop yield
37
negatives of growing genetically modified crops
transplanted genes may get out into the environment genetically modified plants may affect food chains or even human health may create unforeseen problems that will then get passed onto future generations
38
what is micropropagation used for
cloning plants
39
micropropagation process
1. select plant with desirable characteristics 2. small pieces (explants) are taken from the tips of the stems and side shoots 3. explants are sterilised to kill unwanted microorganisms 4. explants grow in vitro - placed in petri dish with nutrient medium and growth hormones 5. cells in explants divide and grow into a small plants 6. small plants are taken out of the medium and planted in soil until they develop into plants which are genetically identical to original plant
40
cloning animals example
Dolly the sheep in 1996
41
cloning animals process
1. nucleus is removed from egg cell of sheep A, creating an enucleated cell 2. A diploid nucleus from a body cell of sheep B is inserted into enucleated cell 3. cell is stimulated using electricity to divide by mitosis 4. dividing cell is implanted into the uterus of sheep C where it develops 5. a clone of sheep B is created
42
how can cloned transgenic animals produce human proteins
cows and sheep make protein naturally in their milk. By transferring human genes into the cells of these animals, they can produce useful human proteins in their milk e.g. they can produce human antibodies which can help disease transgenic chickens can produce human proteins in egg white these animals can then be cloned so the useful genetic characteristic is definitely passed on (doesn't always happen with breeding)