Protein Synthesis Flashcards

1
Q

What are proteins made of

A

Amino acids

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2
Q

Codon

A

Each amino acid is coded for by a sequence of three bases in a gene - this is called a codon

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3
Q

How many different bases are there

A

4:
Adenine (A)
Cytosine (C)
Guanine (G)
Thymine (T)

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4
Q

What does each gene contain

A

A sequence of different bases - which is what allows it to code for a particular protein

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5
Q

Two stages of making protein

A

Transcription
Translation

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6
Q

Where does transcription occur

A

Nucleus

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7
Q

Transcription process

A
  1. DNA is in a double helix
  2. This is separated by the enzyme DNA helicase
  3. There is a template strand and a non-template strand
  4. The strands are left separated and the bases are exposed
  5. There are free RNA nucleotides within the nucleus
  6. Free RNA nucleotides align with template strand due to complementary base pairings
  7. The enzyme RNA polymerase forms sugar phosphate backbone in new mRNA molecule
  8. mRNA detaches from strand and leaves the nucleus to go to cytoplasm
  9. DNA left behind recoils back into a double helix
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8
Q

Where does translation occur

A

In cytoplasm in ribosomes

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9
Q

Translation process

A
  1. mRNA attaches to tRNA (these float around in cytoplasm and has an anticodon and an amino acid) at the start codon
  2. 1st tRNA enters the ribosome, and has complementary anticodon to codon, and brings in an amino acid
  3. 2nd tRNA enters with anticodon that’s complementary to codon
  4. Peptide bond forms between 2 amino acids
  5. 1st tRNA molecule leaves ribosome
  6. Ribosomes move along mRNA to next codon
  7. Process repeats until stop codon
  8. Ribosome detaches from mRNA
  9. Protein formed
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