Protein Synthesis Flashcards
1
Q
What are proteins made of
A
Amino acids
2
Q
Codon
A
Each amino acid is coded for by a sequence of three bases in a gene - this is called a codon
3
Q
How many different bases are there
A
4:
Adenine (A)
Cytosine (C)
Guanine (G)
Thymine (T)
4
Q
What does each gene contain
A
A sequence of different bases - which is what allows it to code for a particular protein
5
Q
Two stages of making protein
A
Transcription
Translation
6
Q
Where does transcription occur
A
Nucleus
7
Q
Transcription process
A
- DNA is in a double helix
- This is separated by the enzyme DNA helicase
- There is a template strand and a non-template strand
- The strands are left separated and the bases are exposed
- There are free RNA nucleotides within the nucleus
- Free RNA nucleotides align with template strand due to complementary base pairings
- The enzyme RNA polymerase forms sugar phosphate backbone in new mRNA molecule
- mRNA detaches from strand and leaves the nucleus to go to cytoplasm
- DNA left behind recoils back into a double helix
8
Q
Where does translation occur
A
In cytoplasm in ribosomes
9
Q
Translation process
A
- mRNA attaches to tRNA (these float around in cytoplasm and has an anticodon and an amino acid) at the start codon
- 1st tRNA enters the ribosome, and has complementary anticodon to codon, and brings in an amino acid
- 2nd tRNA enters with anticodon that’s complementary to codon
- Peptide bond forms between 2 amino acids
- 1st tRNA molecule leaves ribosome
- Ribosomes move along mRNA to next codon
- Process repeats until stop codon
- Ribosome detaches from mRNA
- Protein formed