Biosafety (LAB)

Question 1

process that kills all forms of microbial life, including bacterial endospores

Answer 1

Sterilization

Question 2

What color of bin is use for Infectious waste/ pathological &
anatomical

Answer 2

Yellow

Question 3

physical method of disinfection that Kills vegetative bacteria

Answer 3

Boiling at 100 deg Celsius in 15 minutes

Question 4

BSC Class: In at front through HEPA to the outside or in to the room through HEPA

Answer 4

Class I

Question 5

fire involves combustible cooking media such as oil

Answer 5

Class K

Question 6

Involves flammable materials

Answer 6

Class B

Question 7

Ideal and most common temperature control for AUTOCLAVE

Answer 7

15 psi pressure and 121°C for
30 minutes

Question 8

Filtration of air

Answer 8

HEPA (High Efficiency Particulate Air)

Question 9

What color of bin is use for radioactive waste

Answer 9

Orange

Question 10

Disinfectants, kill endospores, toxic to human

Answer 10

Aldehydes-

Question 11

higher temperatures than moist heat (160°Cto 180 °C); used to sterilize items such as glassware, oil,petrolatum, or powders

Answer 11

Dry Heat

Question 12

removal of pathogenic microorganisms so items
are safe to handle or dispose of

Answer 12

Decontaimination

Question 13

Dry heat temperature

Answer 13

160°C to 180 °C

Question 14

Match the following bacteria to its biosafety level (BSL)

1. HIV,Hep B, Salmonella
2. Mycobacterium Tuberculosis
3. : Bacillus subtilis and Naegleriagruberi
4. Ebola virus, Chigo-Crimeanhemorrhagicfever

Answer 14

1. BSL-2
2. BSL_3
3. BSL-1
4. BSL-4

Question 15

unlikely to be encountered in a routine clinical lab. Organisms are potentially infectious and transmitted through infectious aerosol

Answer 15

BSL-3

Question 16

Disinfectants at high concentrations; used in soaps at low concentrations

Answer 16

Phenolics

Question 17

requires longer exposure times (1.5to3hours)

Answer 17

Dry Heat

Question 18

The important actions in case of fire and the order in which to perform tasks can be remembered with the
acronym RACE:

Answer 18

1. Rescue any injuredindividuals.
2. Activate the fire alarm.
3. Contain (smother) the fire, if feasible (close fire doors).
4. Extinguish the fire, if possible

Question 19

a device that encloses a workspace in such a way as to protect workers from aerosol exposure to infectious disease agents

Answer 19

Biosafety Cabinet

Question 20

Filtration of liquid is done through the use of ____

Answer 20

cellulose acetate / cellulose nitrate membrane

Question 21

disinfectant; toxic at
high levels

Answer 21

Mercuric Chloride

Question 22

Steam under pressure; used to sterilize biohazardous trash and heat- stable objects

Answer 22

Moist Heat

Question 23

long wavelength and low energy

Answer 23

Non-ionizing radiation (UV)

Question 24

Decontamination of inanimate objects or surfaces; Preparation of patients for invasive procedures

Answer 24

Antiseptics

Question 25

Temperature and duration for Pasteurization

Answer 25

70 °C for 30 mins

Question 26

agents are those most commonly being sought in clinical specimens and used in diagnostic, teaching, and other laboratories

Answer 26

BSL-2

Question 27

kills food pathogens w/o damaging nutritional value or flavor

Answer 27

Pasteurization

Question 28

fire involves energized electrical equipment

Answer 28

Class C

Question 29

Supply air is HEPA-filtered. Exhaust air passes through two HEPA filters in series and is exhausted to the outside via a hard connection. Glove ports for attaching arm-length gloves to cabinet.

Answer 29

CLASS III

Question 30

fires involve combustible metals

Answer 30

Class D

Question 31

Involves combustible materials such as wood, paper, cloth, rubber, trash, and plastics

Answer 31

Class A

Question 32

Sterilization of heat-sensitive objects

Answer 32

Gases (Ethylene oxide)

Question 33

What color of bin is use for sharps

Answer 33

Red

Question 34

30% recirculated, 70% exhausted. Exhaust cabinet air must pass through a dedicated,internal cabinet duct to the outside through a HEPA filter

Answer 34

Class II, B

Question 35

Hazardous material burned to ashes at temperatures of 870°C to 980°C; Recommended and safest method

Answer 35

Incineration

Question 36

BSC: 70% recirculated to the cabinet work area through HEPA; 30% balance can be exhausted through HEPA back in to the room or to outside through a canopy unit

Answer 36

Class II, A

Question 37

disinfect drinking water; surface disinfectants

Answer 37

Chlorine and chlorine compounds-

Question 38

agents include those that have no known potential for infecting healthy people and are well defined and characterized.

Answer 38

BSL-1

Question 39

usually use as an eye drop solution.;for prophylactic treatment to prevent gonoccocal conjunctivitis in newborns

Answer 39

Silver Nitrate

Question 40

A process that destroys pathogenic organisms, but not necessarily all microorganisms, endospores, or prions

Answer 40

Disinfection

Question 41

method of choice for antibioticsolutions,toxic chemicals, radioisotopes, vaccines,and carbohydrates, which are all heat sensitive

Answer 41

Filtration

Question 42

What color of bin is use for Chemical Waste, Pharmaceutical waste

Answer 42

YELLOW W/ BLACK BAND

Question 43

Incineration temperature

Answer 43

870°C to 980°C

Question 44

designed to remove organisms larger than 0.3umfrom isolation rooms, operating rooms, and biologic safety cabinets (BSCs)

Answer 44

HEPA

Question 45

What color of bin is use for general/biological waste

Answer 45

Green

Question 46

agents are exotic agents that are highly contagious and cause life threatening disease. No vaccine or therapy available

Answer 46

BSL-4