General Practice Test

Question 1

product of transcription

Answer 1

mRNA strand

Question 2

sterilization process that should be employed for laboratory culture medium

Answer 2

moist heat

Question 3

product of replication

Answer 3

Two complementary DNA strands

Question 4

True or False: The pentose phosphate pathway is the alternative to Entner-Doudoroff
pathway by some heterolactic bacteria like Brucella species

Answer 4

False (Its an alternative to EMP)

Question 5

True or False: Substrate level phosphorylation involves the donation of phosphate from the substrate to ADP.

Answer 5

True

Question 6

True or False: Entner-Doudoroff Pathway is a metabolic pathway utilize by oxidative
organisms to utilize glucose in the formation of ATP

Answer 6

true

Question 7

precursor metabolite for initiating pentose phosphate pathway

Answer 7

Glucose

Question 8

The spores of Bacillus subtilis var. globigii should be killed to determine the efficacy of which sterilant

Answer 8

Ethylene oxide

Question 9

True or False: Alcohols are effective even in the presence of organic material.

Answer 9

False

Question 10

True or False: Phenolics are components of germicidal soaps

Answer 10

True

Question 11

Proposed the practice of washing hands with chlorinated lime solutions

Answer 11

Ignaz Philipp Semmelweis

Question 12

True or False: In establishing the case definition, the microbiology laboratory must first isolate the only causative organism involve in the outbreak

Answer 12

True

Question 13

true or False: An epidemiologic curve is an important visual aid in outbreak investigation

Answer 13

True

Question 14

True or False: Previous unrecognized cases, even if they fit the case definition shall not be included in this outbreak if they did not occur at the time of the outbreak

Answer 14

false

Question 15

How do we sterilize test tubes, volumetric pipets and glass slides in the laboratory

Answer 15

dry heat

Question 16

It remains active in the presence of organic matter and does not coagulate
protein material.

Answer 16

glutaraldehyde/ peraceticacid

Question 17

True or False: Clostridium perfringens is an organism whose toxins cause diarrhea
associated with health care infections.

Answer 17

False

Question 18

true or False: Enterococci are pathogens of
concern in HAIs because of their possible resistance to vancomycin and their
potential ability to pass that resistance on to other bacteria.

Answer 18

True

Question 19

True or False: mbulatory
care settings include a variety of different locations thus in these settings,
commonly encountered microorganisms of infection control importance also
varies.

Answer 19

False

Question 20

bacteria that may employ glycolysis to convert metabolites

Answer 20

E. coli

Question 21

True or False: The product of transduction is a recombination of the DNA of cells
previously infected by the phage.

Answer 21

True

Question 22

True or False: : In plasmid conjugation, all of the plasmids
are transferred via the intercellular bridge

Answer 22

False (transferred through conjugative bridge)

Question 23

Method of sterilization for HEPA filters

Answer 23

formaldehyde gas

Question 24

Capable of self-replication

Answer 24

Episomes

Question 25

structure that is only present in Gram negative bacteria only

Answer 25

Porins

Question 26

S. epidermidis is a bacterium that is part of the skin's microbiota. The host - relationship with this organism can be described as

Answer 26

Symbiotic

Question 27

An intermediate metabolite in the two major metabolic pathways.

Answer 27

Pyruvate

Question 28

Common target of antimicrobial activity

Answer 28

Cell wall

Question 29

True or False: The origin of replication is an area determined by initiation proteins containing approximately approximately 500 base pairs.

Answer 29

False (300 base pairs)

Question 30

True or False: An anticodon consists of triplets of nucleotide bases that encodes for a specific amino acid for binding with the mRNA

Answer 30

False (codon)

Question 31

True or False: Production of antisense strand is a method for silencing genes.

Answer 31

True

Question 32

final step in translation

Answer 32

ribosomal site A encounters UAG codon

Question 33

Certain bacteria are capable of colonization due to

Answer 33

Capsule

Question 34

True or False: S. aureus wound infection of a diabetic person is a manifestation of an opportunistic infection.

Answer 34

True

Question 35

True or False: If person A is positive for the SARS-CoV-2 virus and is also symptomatic, person A can be considered a carrier

Answer 35

False (Carriers Harbors the organism but do not present symptoms to the disease)

Question 36

An acute carrier of S. typhi infection can live for years without manifesting the infection.

Answer 36

True

Question 37

first step in PCR

Answer 37

nucleic acid extraction

Question 38

Kanamycin is ineffective in this group of microorganisms.

Answer 38

Anaerobic Gram positive bacteria

Question 39

Does not induce MLSB methylase formation

Answer 39

Ketolides (telithromycin)

Question 40

Drugs not affected by resistance mechanisms that affect other drug classes

Answer 40

SXT

Question 41

Fluoroquinolones targets which enzyme in Gram negative

Answer 41

topoisomerases II

Question 42

A pivotal test in the identification schemes of Gram positive bacteria

Answer 42

Catalase test

Question 43

What is the purpose of phenol in AFB staining?

Answer 43

Phenol stains the AFB bacilli red.

Question 44

A key test in differentiating Pseudomonas species from Enterobacteriaceae group.

Answer 44

Oxidase test

Question 45

C. acnes is a Gram positive anaerobe. What antibiotics would be effective for this microbe

Answer 45

Lincosamides (clindamycin and lincomycin)

Question 46

The drug binds to the cytoplasmic membrane and inserts its hydrophobic tail into the membrane, disrupting the cell membrane and increasing its permeability, specifically for Gram positive

Answer 46

daptomycin

Question 47

Its ability to disrupt cell membrane is also toxic to human cells.

Answer 47

polymyxin

Question 48

Mucoid colonies are associated with

Answer 48

capsule production

Question 49

Anaerobes requires an environment devoid of oxygen for growth. Anaerobes are examples of

Answer 49

Non-fastidious bacteria

Question 50

The appearance of any species of Mycobacteria using a Kinyoun method of staining:

Answer 50

Red bacilli against a blue background

Question 51

This is considered the differential step in Gram staining

Answer 51

Decolorization process with Acetone/Alcohol

Question 52

Only Pseudomonas aeruginosa may be resistant to the combined action of these antimicrobial agents.

Answer 52

folic acid pathway inhibitor

Question 53

Why is vancomycin ineffective against Gram negative bacteria?

Answer 53

They are unable to penetrate the cell membrane of Gram negative bacteria due to their large molecular size

Question 54

This is a type of antimicrobial employed considered more effective against organisms that are more difficult to control in combination with the host’s immune system.

Answer 54

Bactericidal

Question 55

Treatment for tuberculosis infection

Answer 55

RNA synthesis inhibitor

Question 56

Downregulation of bacterial DNA supercoiling in Gram negative bacteria

Answer 56

fluoroquinolones

Question 57

This antibiotic is highly active against a wide variety of gram-negative and grampositive bacteria; however, its use is limited becauseof its toxicity

Answer 57

Chloramphenicol

Question 58

20% sulfuric acid is an alternative decolorizer in which staining method?

Answer 58

Kinyoun stain

Question 59

Blocks the P-site in translation

Answer 59

linezolid