BIOTECHNOLOGY Flashcards Preview

#‼️BIOCHEMISTRY - PLE TOPNOTCH 2020 > BIOTECHNOLOGY > Flashcards

Flashcards in BIOTECHNOLOGY Deck (10)
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1

Molecular biology lab procedure that is used to synthesize many copies of a desired fragment of DNA

POLYMERASE CHAIN REACTION

2

detects DNA

SOUTHERN BLOT

3

detects mRNA

NORTHERN BLOT

4

detects protein

WESTERN BLOT

5

A rapid immunologic technique testing for antigen-antibody reactivity

ELISA

6

• Fluorescent probe binds to specific gene site of interest

• Specific localization of genes and direct visualization of anomalies (e.g., microdeletions) at molecular level

FISH

• Fluorescence in situ hybridization

7

• Inherited difference in the pattern of restriction

• Important in understanding various single-gene and multigenic diseases

• Useful as a diagnostic tool for diseases involving single-base changes, or deletions/insertions of DNA into a restricition fragment

RESTRICTION FRAGMENT LENGTH POLYMORPHISM

8

• A sequence of duplex DNA that is the same when the two strands are read in opposite directions

• Example:

5’-AGATCT-3’ 3’-TCTAGA-5

PALINDROME

9

• Small, circular, duplex DNA molecules whose natural function is to confer antibiotic resistance to the host cell

PLASMIDS

10

• Hallmark of a bacterial defense system that forms the basis for genome editing technology

• Can be programmed to target specific stretches of genetic code and to edit DNA at precise locations

• With these systems, researchers can permanently modify genes in living cells and organisms and, in the future, may make it possible to correct mutations at precise locations in the human genome in order to treat genetic causes of disease.

CRISPR

• Clustered Regularly Interspaced Short Palindromic Repeats