Chapter 6 Flashcards
(78 cards)
Light microscope
Visible light passed through the specimen and then through the lenses.
Magnification
Ration of objects image to real size
Resolution
Is the measure of clarity of the image ; minimum distance of two points can be separated.
-inversely proportional to wavelength
Contrast
Is the difference in brightness between light and dark area of an image
Organelle
The membrane enclosed structure I. An eukaryotic cells.
Electron microscopes
Focuses a beam of electron through the specimen or onto its surface
Scanning electron microscope
Scans the surface of the sample,usually coated with thin film of gold.
Transmission electron microscope
Used to study internal structure of cell
-the specimen has to be stained with atom of heavy metal
Advantage and disadvantage of electron microscope
-increases resolution But kills the cell
Cell fractionation
A useful study that is used to study cell structure and function. It take ps cell parts and separate major organelle and other sub cellular structure from one another. Sample in spin in centrifuge and separated according to density. Larger cellular components are in the pellet while small components are in pellets after high speeds.
Eukaryotic cell
DNA is bounded within the nucleus
Eukaryotic cell
DNA is concentrated in a region not bounded in membranes enclosed organelle like nucleus
Nucleoid
Area where DNA is concentrated
Cytoplasm
The interior of either type of cell . . I’m eukaryotic cell it’s is between nucleus and
Plasma membrane. Within cytoplasm there is cystic in which organism are dissolved.
Plasma membrane
Boundary of all living cell is called plasma membrane.it acts as selective barrier that allows passage of enough nutrients and waste to service entire cell.
How surface area and volume changes as size increases.
As cell size increases , surface area grow volume decrease.
Nucleus
It contains most of the genes in the eukaryotic cell. It is generally 5um
Nuclear envelope
Encloses the nucleus separating from cytoplasm plasma. Double membrane .
Nuclear lamina
A netlike array of Protein filament that maintain the shape of the nucleus by mechanically supporting the nuclear envelope.
Chromosomes
Within the nucleus, DNA is organised into discrete subunit called chromosomes
Chromatin
The complex of DNA and protein making up chromosomes is called chromatin
Nucleolus
A prominent structure within nucleus. A
Under microscope appear as mass dense stained granules
Ribosome
Complex made of ribosomal RNA and protein, are cellular components that carry out protein synthesis.higher the rate of protein synthesis more the ribosome
Smooth ribosome
- are suspended in the systol
- also called free ribosome