Clearing

Question 1

Process whereby alcohol or the dehydrating agent is removed from the tissue and replaced with substance that will dissolve the wax with which the tissue is to be impregnated

Answer 1

Clearing (dealcoholization)

Question 2

What is the most common clearing agent?

Answer 2

xylene

Question 3

What clearing agents are used when the tissue is to be cleared directly from water (frozen section)?

Answer 3

Glycerin and gum syrup

Question 4

Clearing time of xylene.

Answer 4

1⁄2 (30 mins.) to 1 hour

Question 5

What will happen to the tissue if it was cleared for greater than 3 hours in a xylene solution?

Answer 5

It will become hard or brittle.

Question 6

What clearing agent is used to replace benzene and xylene with 1-2 hours of clearing time?

Answer 6

toluene

Question 7

What clearing agent may cause aplastic anemia?

Answer 7

benzene

Question 8

What clearing agent is used for large tissue specimens with a sleeping effect?

Answer 8

chloroform

Question 9

What clearing agent is recommended for central nervous system tissues, and cytological studies, particularly of smooth muscle and skin?

Answer 9

cedarwood oil

Question 10

How many times will you change a solution immersed in cedarwood oil?

Answer 10

2

Question 11

Clearing time of cedarwood oil.

Answer 11

2-3 days

Question 12

What clearing agent is used for clearing embryos, insects, and delicate specimens?

Answer 12

aniline oil

Question 13

What clearing agent has highly toxic effects on the respiratory system?

Answer 13

carbon tetrachloride

Question 14

What clearing agent has the ability to perform two processes at the same time with the non-toxic and offensive odor?

Answer 14

tetrahydrofuran

Question 15

What clearing agent has toxic effects on the human liver?

Answer 15

dioxane

Question 16

How many changes are made when using dioxane?

Answer 16

3 within 4 hours

Question 17

What clearing agent is an isoprene polymer found in essential oils derived from plants?

Answer 17

Terpenes

Question 18

What clearing agent from the terpene family is a volatile oil found in citrus peels?

Answer 18

limonene

Question 19

What clearing agent from the terpene family offers the clearing action with the lowest hazard rating of all xylene alternatives?

Answer 19

oranges oil-based clearing agents

Question 20

What clearing agent from the terpene family are restricted by the government regulations?

Answer 20

chlorinated hydrocarbons

Question 21

What clearing agent acts as a dewaxing agent during staining?

Answer 21

conventional xylene with bleached palm oil

Question 22

What clearing agent has the tendency to get solidified at a lower temperature?

Answer 22

coconut oil