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Flashcards in Complete blood count Deck (36)
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1

Flow Cytometry - what is it and how it works

Hydrodynamic focusing and passes cells through probe in single file line --> voltage drop --> relates to cell size and counts cells

2

Units of RBC count, HGB, HCT, WBC

x10^6/uL or 10^12/L
g/dL or g/L
Percent
thousands per uL or millions per L

3

Define MCV

mean size of red cells femptoliters

4

Define MCH

mean corpuscular hemoglobin (amount of hemoglobin in a single red cell) pg

5

Define MCHC

Average concentration of hgb in cell (g/dL or g/L0

6

Equation to find MCHC

MCHC = HGB/HCT

7

Equation for MCV

MCV = HCT/RBC x 100

8

Morphology: neutrophils

9-15 um
cytoplasm acidophilic
very fine granules
2-5 nuclear segment/lobes
WBC: 4 - 11.1
N% 39.7 - 72.11
N: 1.8-6.6

9

Morphology: Eosinophils

12-17 um
Numerous large, round, red-orange granules
1-4 nuclear lobes (typically 2)

Note (Stimu by IL5) - parasite killers

10

Morphology Basophil

12 um
Numerous large/round purple black bytoplasmic granules
2 nuclear lobe - may be covered by granules

11

Morphology Monocyte

15-20 uM
Large eccentric nucleus, round kidney/horseshoe shaped/lobulated
Chromatin lacy appearance
Abundant cytoplasm, azurophilic granules
intracytoplasmic vacuoles

12

Morphology Lymphocyte

7-12 um
Condensed chromatin
Scanty bluish cytoplasm, few azurophilic granules
nucleus round/slightly indented (cell mostly nucleus)

13

Morphology platelets

small 2-3 um
7-8 fl
irregular outline
light blue cytoplasm
small azurophilic granules

14

Morphology - iron deficiency anemia

RBC small - large central pale area, target cells

15

Morphology spherocytes

spherical, no central pallor, decrease cell membrane, increase MCHC
Immune hemolytic anemia, hereditary spherocytosis

16

Morphology bite cells

bite like detect - removal of heinz body in spleen
G6PH deficiency

17

Schistocytes morphology

Fragmented RBC, helmet cells
Burns, HUS, TTP, DIC, HELLP, mechanical heart valves

18

Target cell morphology

Central hemoglobin, target shape
Thalassemia, hemoglobin C, iron deficiency, liver disease

19

Sickle cell

Bannana shape

20

Basophilic stippling

Evenly dispersed fine blue granules in RBC - aggregated rNRA
Lead poisoning, porphyria, pryimidine 5' nucleotidase deficiency
Hemoglobinopathies, thalassemia
Myelodysplasia, sideroblastic anemia
Infection

21

Howell Jolly Bodies

Single dense, blue dot
Nuclear DNA remnant
Post-splenectomy, functional asplenia , megaloblastic anemia, myelodysplasia

22

Heinz body

Not visible on wright-diemsa stain - need to stay with supravital dye/crystal violet
Denatured/oxidized hemoglobin attached to inner cell membrane --> G6PH deficiency
Associated with bite cell

23

Dohl Body

WBC - pale blue inclusion at periphery of cytoplasm
infection, inflammation, burns, pregnancy
condensed RNA

24

Toxic granulation/hypergranularity

WBC - increase number and primary granules - due to trapid cell division
Bacterial infection, marrow recovery, GM-CSF

25

Hypersegmented neutrophils

More than 5 lobes, megaloblastic anemia

26

Microcytic/hypochromatic RBC

RBC smaller with larger central pale area - iron deficiency anemia

27

Elliptocytosis

elliptical - defective spectrin alpha chain

28

Ovalocyte

large, ovoid RBC - B12/folate deficiency

29

teardrop cells

pear shaped - myelophthistic changes

30

Stomatocyte

elongated central pallor
alcohol, dilantin, Rh null, hereditary stomatocytosis