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Flashcards in Antibody Deck (40)
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1

H chain - define

heavy chain - 1 variable region 3-4 constant region

2

L chain - define

light chain - 1 variable region 1 constant region

3

Kappa chains - define

Type of L chain

4

Lamda chains - define

type of L chain

5

Fab - define

Fragment antibody - contains the variable L/H + CL/H (only one control domain

6

Fc - define

Fragment constant - only the heavy chains' CH2, 3, (4)

7

Put in order on electrophretic separation:
Anode, cathode, albumin, alpha 1, alpha 2, beta, gamma peaks

Cathode | albumin, alpha 1, alpha 2, beta 1, beta 2, gamma | Anode

8

Heavy chain for IgG, IgE, IgD, IgA, IgM

G - 2 gamma chains
E - 2 epsiolon CH4 (4th domain) - affinity for mast cells (histamine) - allergies
D - 2 delta chains for baby cells - receptor molecule - note extra long hinge
A: 4 alpha chains, 1 J, and 1 S.C. chain
M: 10 mu, 1 J ring/chain

9

Hinge

Between Fab/Fc - can change conformation

10

Ig_ has secretory chain

IgA

11

Order immunoglobins classes from largest to smallest

M, A, E, D, G

12

Antibody combining site

3 CDR on each light/heavy chains
Once bound --> allosteric changes. Fc changes structurally

13

CDR

complementary determining region - also hypervariable region - random splicing

14

Allotype

minor allectic difference in sequence of immunoglobulins (ie. Ala vs Tyr on specific spot on heavy chain)

15

Idiotype

antibody with unique CDR - if sequence is recognized by another antibody- will behave as an antigen (Ab behaves as an Ag)

16

IgG is ___ valent?

Di

17

Immune complex can happen when:

antibody cross linking two antigen

18

Aggregation --> fall out of solution. Antigen is a molecule, aggregation is called ______; antigen is a cell, aggregation is called a ____

precipitation, agglutination

19

Ig_ is made by fetus

M

20

Ig_ cross placenta via _________________

IgG --> placenta via active transport

21

Ig_ is decavalent, can activate complement with _#_ antibody

M
1

22

Ig_ needs __ antibody to complement

G, 2

23

IgG has half life of...

3 weeks

24

Phagocytic cells have FcR for Ig_ --> opsonizing

IgG

25

Ig_ is most common in blood

G

26

Ig_ in mucosa

A

27

Ig_ is first line of immunological defense

A

28

Ig_ for B cell receptors

D

29

Ig_ is responsible allergies/hypersensitivity

E

30

Ig_ is for ____ cells

mast/basophils (have FcR for IgE) --> histamines