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Flashcards in Conventionally Cultured Bacteria Deck (72)
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What were the two Gram +ve aerobic cocci groups covered in class?

Staphylococcus and streptococcus

1

List 3 Major distinguishing features of Staphylococcus

1. Gram +ve
2. Cocci in clusters
3. Catalase +ve

2

What two Staphylococci species were presented?

1. S. aureus
2. Coagulase negative Staphylococci (CNS)

3

What are two virulence factors of S. aureus?

1. Toxic Shock Syndrome Toxin 1 (TSST-1)
2. Enterotoxins

4

What 7 clinical presentations is S. aureus associated with?

1. SSTI
2. Necrotizing fasciitis
3. Osteomyelitis
4. Endocarditis
5. Toxic shock syndrome
6. Cystic fibrosis pneumonia
7. Diarrhea

5

Primary pathogenicity of CNS, such as S. epidermidis?

Biofilms

6

Clinical significance of CNS (2)

1. Nosocomial bloodstream infections from IV catheters
2. Prosthetic material infections

7

3 major distinguishing features of Streptococcus species

1. Gram +ve
2. Cocci in pairs and chains
3. Catalase -ve

8

List all Streptococcus species presented in class (5)

1. Group A Streptococcus (S. pyogenes)
2. Group B Streptococcus (S. agalactiae)
3. S. pneumoniae
4. S. mitis
5. Enterococci

9

What are the 2 B-hemolytic Strep species presented?

1. GAS (S. pyogenes)
2. GBS (S. agalactiae)

10

What are the 2 a-hemolytic strep species that were presented?

1. S. pneumoniae
2. S. mitis

11

What was the only non-hemolytic strep species presented?

Enterococci

12

Pathogenicity of GAS

Streptolysin O and S

13

Pathogenicity of GBS

Polysaccharide capsule

14

Pathogenicity of S. pneumoniae

Polysaccharide capsule

15

Pathogenicity of S. mitis

Polysaccharide capsule

16

Pathogenicity of enterococci

Surface adhesins

17

Diseases associated with GAS (4)

1. Pharyngitis
2. Necrotizing fasciitis
3. Rheumatic fever
4. Streptococcal Toxic Shock Syndrome (STSS)

18

Diseases associated with GBS (2)

1. Early onset neonatal disease (pneumonia, sepsis)
2. Late onset neonatal disease (meningitis, sepsis)

19

Diseases associated with S. pneumoniae (4)

1. Community acquired pneumonia
2. Otitis media
3. Conjunctivitis
4. Meningitis

20

Diseases associated with S. mitis (2)

1. Endocarditis
2. Bacteremia

21

Diseases associated with enterococci (3)

1. UTI
2. Endocarditis
3. Nosocomial VRE

22

Aerobic Gram +ve bacilli groups presented (2)

1. Corynebacterium
2. Listeria

23

Major features of Corynebacterium (4)

1. Gram +ve
2. Rods
3. Non-branching
4. Catalase +ve

24

2 examples of Corynebacterium

1. C. diphtheriae
2. C. jeikeium

25

Pathogenicity and disease associated with C. diphtheriae

1. Diphtheria toxin
2. Diphtheria

26

C. jeikeium has unknown pathogenicity, but causes this type of infection(s)

Foreign body infections

27

5 features of Listeria

1. Gram +ve
2. Single or short chain rods
3. Non-branching
4. Motile
5. Catalase +ve

28

Pathogenicity of L. monocytogenes (1)

1. Hemolysin

29

6 problems caused by L. monocytogenes

1. Gastroenteritis
2. Meningitis
3. Encephalitis
4. Abortion
5. Stillbirth
6. Preterm labour

30

Anaerobic Gram +ve cocci genus presented (1)

Peptostreptococcus

31

3 features of Peptostreptococcus

1. Elongated cocci
2. Gram +ve
3. Non-spore forming

32

Pathogenicity of Peptostreptococcus is unknown, what is its clinical significance? (4)

1. Cutaneous
2. Respiratory
3. Oral
4. Female pelvic infections
Often mixed infections

33

Anaerobic Gram +ve bacilli (2 genus)

1. Actinomyces
2. Clostridium

34

2 features of Actinomyces

1. Gram +ve
2. Branching, filamentous bacilli

35

Actinomyces israelii has unknown pathogenicity and causes 2 things

1. Cervicofacial infections
2. Pelvic infections (IUD)

36

5 features of Clostridium spp.

1. Gram +ve
2. Box car rods = C. perfringens
3. Straight rods = C. difficile, C. tetani
4. Straight/curved rods = C. botulinum
5. Spores

37

4 Clostridium species

1. C. tetani
2. C. difficile
3. C. perfringens
4. C. botulinum

38

2 toxins made by C. perfringens

1. a - toxin
2. Enterotoxin

39

3 diseases associated with C. perfringens

1. Gas gangrene
2. Bloody diarrhea
3. Acute necrotizing GI infection

40

2 toxins and disease caused by C. difficile

1. Toxin A
2. Toxin B
3. Diarrhea

41

2 genus of aerobic Gram -ve fermenters mentioned in class

Enterobacteriaceae
Vibrio spp.

42

The group Enterobacteriaceae includes the following 4 organisms

1. E. coli
2. Enterobacter
3. Salmonella
4. Proteus

43

3 features of Enterobacteriaceae

1. Gram -ve
2. Rods
3. Oxidase -ve

44

Virulence factors of E. coli (4)

1. Endotoxin
2. Exotoxins
3. Capsule
4. Pili

45

3 infections caused by E. coli

1. Meningitis in newborns
2. UTI
3. GI infection

46

3 pathogenic factors of Enterobacter spp.

1. Exotoxins
2. Endotoxins
3. Capsules

47

3 diseases associated with Enterobacter spp.

1. UTI
2. Nosocomial infections
3. Blood infections

48

Pathogenicity of Salmonella spp. (3)

1. Adhesion proteins
2. Survival in phagocytes
3. Dissemination to other tissues

49

2 common infections associated with Salmonella spp.

1. GI infections
2. Bacteremia

50

Pathogenicity of Proteus spp. (4)

1. Exotoxins
2. Endotoxins
3. Capsules
4. Adhesion proteins

51

3 infections associated with Proteus spp.

1. UTI
2. Nosocomial infections
3. Blood infections

52

3 major features of Vibrio

1. Gram -ve
2. Rods
3. Oxidase +ve

53

Pathogenicity of V. cholerae (2)

1. Somatic O antigens
2. Exotoxins

54

Aerobic Gram -ve non-fermenters shown in class include these 2 groups

1. Moraxella
2. Pseudomonas

55

3 major features of Moraxella

1. Gram -ve
2. Diplococci
3. Oxidase +ve

56

Moraxella catarrhalis has which pathogenicity and causes what disease...

1. Uncertain, but has cell envelope adhesion factors
2. Community acquired pneumonia

57

Major features of Pseudomonas (5)

1. Gram -ve
2. Slender bacilli
3. Oxidase +ve
4. Metallic sheen
5. Grape-like odor

58

Pseudomonas aeruginosa has exotoxins and endotoxin - what infections is it associated with? (4)

1. Otitis externa
2. Pneumonia in cystic fibrosis
3. Ventilator acquired pneumonia
4. UTI

59

Haemophilus and Neisseria belong to which category of pathogen we learned about?

Fastidious Gram -ve bacilli/cocci/coccobacilli

60

4 features of Haemophilus spp.

1. Gram -ve
2. Coccobacilli or short rods
3. Satellite
4. Oxidase +ve

61

2 virulence factors of Haemophilus influenzae

1. Capsule
2. Outer membrane protein

62

5 clinical presentations of H. influenzae infection

1. Meningitis
2. Epiglottitis
3. Conjunctivitis
4. Otitis media
5. Pneumonia

63

3 features of Neisseria spp.

1. Gram -ve
2. Diplococci
3. Oxidase +ve

64

Genus Neisseria includes these 2 species

1. N. meningitidis
2. N. gonorrhea

65

Pathogenicity of N. meningitidis (2)

1. Capsule
2. Outer membrane protein

66

Pathogenicity of N. gonorrhea (4)

1. Pili
2. Capsule
3. Proteases
4. Adherence proteins

67

3 clinical presentations of N. gonorrhea

1. Gonorrhea
2. Pharyngitis
3. Conjunctivitis

68

Bacteroides is a "BLANK" Gram "BLANK"

Anaerobic, negative

69

Pathogenicity of Bacteroides fragilis (3)

1. Capsule
2. Endotoxin
3. Enzymes

70

Clinical significance of Bacteroides fragilis (3)

1. Abscesses throughout body
2. Bacteremia
3. Aspiration pneumonia

71

"BLANK" is the only genus of aerobic gram -ve fermenters shown in class

Vibrio