effect of ph on amylase

Question 1

stage 1

Answer 1

place 1 drop of iodine solution into each well of a spotting tile

Question 2

stage 2

Answer 2

take 3 test tubes filled with 2cm^3in each: starch solution, amylase sol and buffer sol

Question 3

stage 3 why

Answer 3

place all in a waterbath at 30*c
leave for 10 mins to allow the sols to reach the correct temp

Question 4

stage 4

Answer 4

comebine all 3 sols into one test tube and mix with a stirring rod

Question 5

stage 5

Answer 5

return mixed sol into a waterbath and start a stopwatch

Question 6

stage 6

Answer 6

after 30s use the rod to transfer one drop of the mixed solution to an iodine well

Question 7

stage 7

Answer 7

iodine should be blue black showing starch is present
keep taking a sample every 30s until iodine remains orange

Question 8

stage 8

Answer 8

repeat using different pH buffers

Question 9

problem: time
why

Answer 9

samples taken every 30s only - only approx time for reaction to complete

Question 10

how can we address problem of time

Answer 10

take samples over shorter intervals like 10 seconds

Question 11

problem: colour
why and how addressed

Answer 11

looking for time the iodine isnt blue black - not aways obvious
addressed by asking for others opinions