Endomembrane System Part I

Question 1

transport vesicles

Answer 1

• exchange materials bewteen organeles

Question 2

donnor and acceptor membrane compartments

Answer 2

• Donor compartment budding
• visiaes
• recpeoient compartment fusion

Question 3

vesicle coat proteins

Answer 3

• Select which donor membrane and soluble cargo proteins enter
• regulate vesicle formation

Question 4

biosythesis pathway

Answer 4

er -> Golgi -> endosome ->lysosome

Question 5

secretory pathway

Answer 5

1: constititive pathway
- materials continualy transpoeted from golgi to PM or released
2: regulated secretion
- in specialized cells
-er derived materials from golgi stored in secretory granuels
- granuels fse with pm and release lumenal cargo

Question 6

Endocytic pathway

Answer 6

• paterials frompm and/or extracellular space into cell
• transported to endosomes then lysosomes

Question 7

autoradiography and pulse chase radiolabeling

Answer 7

• Proteins move through secretory pathway
• ose membrane-bound intermediates
• pancreatic tissue, AA

Question 8

live cell imaging using auto fluorescent proteins

Answer 8

gene encoding autoflourent protein linked to gene of interest

Question 9

subcellular fractioning

Answer 9

• seprate purity specific organeles (isolation)
• homogenization

Question 10

microsomes

Answer 10

fragments of ER membrane that fuse and reform into small spherical vesicles

Question 11

equilibium density gradient

Answer 11

• seperates intact organells/cellular compenents on basis of density
• higher density at bottom

Question 12

cell free assays

Answer 12

determine composition of isolated organelles

Question 13

liposomes

Answer 13

• incubate isolated proteins
• similar to micells (phospholibd bilyaer)

Question 14

mutant phenotype analysis

Answer 14

identify gene /protiens

Question 15

yeast ssc mutants