Microscopy Flashcards
(11 cards)
1
Q
brightfield microscopy
A
- poor contrast
- magnification and resolution and specimen fictation/staining
2
Q
deconvolution
A
designed to remove background and out of focus light
3
Q
magnificiation
A
primary focus of microscopy
4
Q
reolution
A
1: wavelength
2: numerical apature - light gathering qualities of objective lens and specimen matinf medium
5
Q
formaldehyde fixation
A
- crosslinks Amino groups on adjacent proteins/nucleic acids
-fixation xan result in cell death and damage
6
Q
microtome
A
???
7
Q
flouresence microscope
A
- visualizes flouresence
- antibodes
- auto flouresence: endogenous flouresnce
- iminoflouresence: aplied dyc antibodies
- auto flouresence protiens
8
Q
confocal laser scanning microscop (CLSM)
A
- more detailed imaging
- specimine usually living
- point laser at specific wavelength
- specimene focused through pinhole
9
Q
CLSM Z section
A
2D slide collected at different depths to create stack and generate image
10
Q
photobleach vs phototoxicity
A
kimitations to clsm
cell death
bleach: no flouresence
toxicity: too much flouresence
11
Q
super resolution CLSM
A
- 10X resolution
- creates cross section at diff wavelength and angles
- takes longer
