ENZYMATIC TECHNIQUES

Question 1

primary granules of granulocytic cells

Answer 1

MYELOPEROXIDASE

Question 2

neutrophils
eosinophils
monocytes
lymphocyte (-)

Answer 2

MYELOPEROXIDASE

Question 3

acute myeloid leukemia (AML) vs. acute lymphoblastic leukemia (ALL)

Answer 3

MYELOPEROXIDASE

Question 4

lysosomes
all hematopoietic cells
T lymphocytes

Answer 4

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Question 5

preliminary identification of T ell subsets of acute lymphoblastic leukemia (ALL)

Answer 5

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Question 6

L-tartaric acid + staining substrate

Answer 6

TARTRATE-RESISTANT ACID PHOSPHATASE

Question 7

hairy cells

Answer 7

TARTRATE-RESISTANT ACID PHOSPHATASE

Question 8

hairy cell leukemia

Answer 8

TARTRATE-RESISTANT ACID PHOSPHATASE

Question 9

diaminobenzidine (DAB) (benzidine substitute)

Answer 9

MYELOPEROXIDASE

Question 10

brown reaction product

Answer 10

MYELOPEROXIDASE

Question 11

copper salts followed by Gill’s modified Papanicolaou stain

Answer 11

MYELOPEROXIDASE

Question 12

intense gray-black granules

Answer 12

MYELOPEROXIDASE

Question 13

neutrophil and monocyte

Answer 13

MYELOPEROXIDASE

Question 14

differentiates cells of lymphoid origin
from granulocytes and their precursors and monocytes

Answer 14

MYELOPEROXIDASE

Question 15

Peripheral blood or bone marrow smears

Answer 15

ACID PHOSPHATASE (ACP) without Tartrate
resistance

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

ALKALINE PHOSPHATASE (ALP

ESTERASES

Question 16

naphthol AS I phosphoric acid and fast garnet GBC salt.

Answer 16

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Question 17

enzymatic hydrolysis

Answer 17

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Erastase

Question 18

insoluble maroon dye

Answer 18

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Question 19

cellular sites

Answer 19

ACID PHOSPHATASE (ACP) without Tartrate
resistance

Question 20

L- tartrate–containing substrate

Answer 20

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

Question 21

Cells containing tartaric acid–sensitive acid phosphatase do not exhibit any dye deposits

Answer 21

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

Question 22

mononuclear cells containing tartaric acid–resistant acid phosphatase are not affected by such treatment

Answer 22

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

Question 23

leukocytes (+)
lymphocytes ()

Answer 23

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

Question 24

tartrate resistant

Answer 24

hairy cell leukemia, Sézary syndrome, and some T-cell acute lymphoblastic leukemias
are

Question 25

ACP IsoE 5

Answer 25

TARTRATE-RESISTANT ACID PHOSPHATASE (TRAP)

Question 26

alkaline-dye solution

Answer 26

ALKALINE PHOSPHATASE (ALP

Question 27

alkaline-dye solution

Answer 27

ALKALINE PHOSPHATASE (ALP)

Question 28

naphthol AS-MX phosphate

Answer 28

ALKALINE PHOSPHATASE (ALP)

Question 29

'fast blue RR salt or fast violet B salt

Answer 29

ALKALINE PHOSPHATASE (ALP)

Question 30

diazonium salt

Answer 30

ALKALINE PHOSPHATASE (ALP) ESTERASES

Question 31

insoluble, visible pigment

Answer 31

ALKALINE PHOSPHATASE (ALP)

Question 32

Leukocyte alkaline phosphatase (LAP) activity

Answer 32

ALKALINE PHOSPHATASE (ALP)

Question 33

leukemoid reactions and chronic granulocytic (myelogenous) leukemia

Answer 33

ALKALINE PHOSPHATASE (ALP)

Question 34

touch preparations

Answer 34

ESTERASES

Question 35

a Naphthol compound

Answer 35

ESTERASES

Question 36

Naphthol AS-D chloroacetate

Answer 36

ESTERASES

Question 37

alphanaphthyl acetate or alpha-naphthyl butyrate

Question 38

stable diazonium salt

Answer 38

ESTERASES

Question 39

highly colored deposits

Answer 39

ESTERASES

Question 40

granulocytic vs. monocytic

Answer 40

ESTERASES

Question 41

differentiation of leukemias

Answer 41

ESTERASES

Question 42

• identify the cells of the granulocytic series. It does not stain lymphocytes and monocytes

Answer 42

Specific esterase (Naphthol AS-D chloroacetate)

Question 43

• It can even be used to identify granulocytes in formalin fixed tissue

Answer 43

Specific esterase (Naphthol AS-D chloroacetate)

Question 44

• In this procedure, blood or bone marrow smears are incubated with naphthol AS-D chloroacetate in the presence of a stable diazonium salt

Answer 44

Specific esterase (Naphthol AS-D chloroacetate)

Question 45

• Naphthol compounds are coupled with diazonium salt, forming highly colored deposits at the sites of enzyme activity

Answer 45

Specific esterase (Naphthol AS-D chloroacetate)

Question 46

• seen in monocytes (see Figure 5

Answer 46

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 47

• Both α-naphthyl butyrate or α-naphthyl acetate can be

Answer 47

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 48

The cells of granulocytic series are negative with this stain

Answer 48

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 49

The α -naphthyl butyrate stain is more specific, while the αnaphthyl acetate stain is more sensitive

Answer 49

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 50

In a suspected myeloid leukemia, dual esterase stains can be used as they can simultaneously classify the blasts as either myeloblasts or monoblasts

Answer 50

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 51

To differentiate positive reacting cells conclusively from monocytes, sodium fluoride is incorporated with the incubation system

Answer 51

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 52

The monocyte enzyme is inactivated in the presence of this compound

Answer 52

Non-specific esterase (Alpha-naphthyl acetate esterase

Question 53

Monocytic non-specific esterase is Fluoride sensitive.

Answer 53

Non-specific esterase (Alpha-naphthyl acetate esterase