Enzyme Practicals

Question 1

Investigate the effect of pH on the rate of reaction of amylase enzyme

Question 2

What reaction does amylase catalyse?

Answer 2

The breakdown of starch to maltose

Question 3

Step 1

Answer 3

A drop of iodine needs to be put into each well of a spotting tile

Question 4

Step 2

Answer 4

Use a water bath to heat a solution of 2 cm^3 pH 7 amylase solution and 1 cm^3 amylase solution and 5cm^3 of starch solution in separate test tubes to a constant temp of 35 degrees celcius.

Question 5

Control

Answer 5

Set up a control experiment without the amylase to make sure that the starch wouldn’t break down anyway - The result should be negative so the colour should remain blue-black

Question 6

Step 3

Answer 6

Add the buffer solution to the amylase solution, then, add the starch solution to the test tube and mix

Question 7

Step 4

Answer 7

Start timing immediately and using a pipette remove one drop of the starch - amylase solution and add it to one of the wells on the spotting tiles

Question 8

Step 5

Answer 8

Wait 30 secs and add another drop to an empty well repeat this, with 30 second intervals each time until the the iodine solution remains yellow-brown

Question 9

Step 6

Answer 9

Record the time that it took. Repeat this 5 more times at a diff pH value each time.

Question 10

How to calculate the rate?

Answer 10

1000/time

Question 11

Hazards

Answer 11

.Amylase can cause allergic reactions
.iodine is irritant avoid contact with skin and eyes

Question 12

Safety measures

Answer 12

. goggles
.gloves

Question 13

How else can you measure rate of reaction?

Answer 13

change/time