(F) Lesson 5: Laboratory Assistant/Laboratory Waste Management Plan Flashcards

(114 cards)

1
Q

T or F: Laboratory assistant have job assignments that vary from one institution to another

A

True

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2
Q

A laboratory assistant may be assigned into which two roles?

A

A. Receptionist in the reception area
B. Laboratory aide in testing areas

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3
Q

T or F: Laboratory glasswares should not only be clean physically but also chemically.

A

True

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4
Q

What is the common two-way rinse of washing laboratory glassware?

A

With tap and distilled/deionized water

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5
Q

How do you commonly dry laboratory glassware?

A

Air dry or with drying oven

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6
Q

In removing grease, you need to boil the glassware in which weak solutions?

A

Sodium carbonate, acetone, or any fat solution

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7
Q

For greasy stopcock in laboratory equipment, in which solution do you soak it and for how long?

A

In a warm decahydronaphthalene for 2 hours

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8
Q

In removing grease, what do you use to rinse it and for how long?

A

With acetone or fuming sulfuric acid for 30 mins

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9
Q

In rinsing, how many times do you fill with water, shake, and empty each test tube, graduated cylinder, and/or flask?

A

6 times

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10
Q

These are machines/a type of technology that can be used for cleansing pipets

A

Pipet washers

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11
Q

T or F: In washing pipets, place pipets with tips up in a cylinder or tall jar of water.

A

False (tips down)

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12
Q

If the pipet is dirty, where do you soak it?

A

In a jar of chromic acid cleaning solution

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13
Q

How long do you soak in distilled water the pipet after rinsing with tap water?

A

At least 1 hour

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14
Q

In washing, the culture tubes are autoclaved for how long?

A

30 mins

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15
Q

In washing, the culture tubes are autoclaved in which temperature and pressure setting?

A

121 deg C and 15 psi

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16
Q

What are used to sterilize Petri dishes and culture bottles?

A

Autoclave or a dry-air sterilizer

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17
Q

In sterilizing serology tubes, what do you add in the bucket before boiling?

A

1% - 2% soap or detergent

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18
Q

In sterilizing serology tubes, how long do you boil them?

A

30 mins

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19
Q

What is the drying temperature for serology tubes and culture media?

A

less than 140 deg C

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20
Q

Where do you hang serology tubes to dry?

A

Wooden pegs

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21
Q

T or F: Acidic liquids should not be stored in flasks because the stoppers or stopcocks may stick

A

False (alkaline)

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22
Q

Where do you document the cleaning and maintenance activities in the laboratory?

A

Laboratory logbook

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23
Q

T or F: The laboratory exit floor plan should have three clear passageways.

A

False (two)

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24
Q

T or F: When designing the location of safety showers, eyewashes, fire extinguishers, etc, aesthetic value should be considered

A

False (must be strategically placed)

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25
T or F: Fire extinguisher should be placed near the door
True
26
What should be maintained and filled with water to avoid sewer gases from escaping?
Sink traps and floor drains
27
What should be placed away from any edges?
Bench apparatus
28
T or F: Cleaning reagents must be prepared at least twice a week
False (daily)
29
T or F: Cleaning reagents is safer and more efficient to store in diluted form
False (should not)
30
What are the labels put in preparing cleaning reagents?
Name and date of preparation
31
Disinfectant used for daily cleaning
1 % sodium hypochlorite solution
32
To prepare 1 % sodium hypochlorite solution, add how many mL of sodium hypochlorite solution to how many mL of water?
10 mL of sodium hypochlorite to 1000mL of water (10:1000)
33
To produce 1 liter of 70% ethanol, how many mL of absolute ethanol shall be added with how many mL of distilled water?
700mL of absolute ethanol with 300mL of distilled water (700:300)
34
1 liter of 5% Lysol is produced by adding how many mL of 12% Lysol to how many mL\ of distilled water?
450 mL of 12% Lysol to 550 mL of distilled water
35
In daily cleaning procedure, which disinfectant is poured and spread on the work surface with paper towel?
5% Lysol
36
What two-way disinfection is used in weekly cleaning of the work area?
Soap solution anf 5% Lysol
37
In weekly cleaning of the work area, which disinfectant do you use when you clean the ultrasonic water bath?
Hypochlorite (bleach) solution
38
In weekly cleaning of the work area, which disinfectant do you use when you clean the racks and tubes?
5% Lysol
39
In weekly cleaning of the work area, which disinfectant do you use when you clean the racks for molecular testing?
1% sodium hypochlorite (bleach solution)
40
Recite the waste management hierarchy pyramid from most preferable to least preferable
Reduce Reuse Recycle Recover Dispose
41
Waste management hierarchy; Minimizing the amount of waste produced
Reduce
42
Waste management hierarchy; using products repeatedly
Reuse
43
Waste management hierarchy; using materials to make new products
Recycle
44
Waste management hierarchy; obtaining energy from waste
Recovery
45
Waste management hierarchy; landfill and incineration without energy recovery
Disposal
46
T or F: The waste management plan should not only focus on minimizing the potential hazardous risk but also consider minimizing the impact of the disposal of waste materials on the environment
True
47
Tier of strategic hierarchy; pollution prevention and source reduction
Green chemistry
48
Tier of strategic hierarchy; used to treat unwanted materials, surplus chemicals, and hazards
Reuse, redistribute, and reduce
49
Tier of strategic hierarchy; recycling chemicals and recover energy from waste
Recycle
50
Tier of strategic hierarchy; refers to incineration, land disposal, and other treatment methods
Environmental fate of the waste
51
Refers to the primary consideration of waste disposal
Safety
52
Refers to the temporary storage for waste in the laboratory
Satellite accumulation area
53
T or F: Waste materials have different categories and corresponding safety precautions and follow an appropriate disposal method
True
54
These are trays used for spills and leakages from any primary container
Secondary containment devices
55
Waste materials in the satellite accumulation should not be kept for how long?
1 year
56
Waste exceeding how many gallons should be managed within 3 days?
55-gal
57
Waste exceeding the 55-gal (or 1-qt) limit should be managed accordingly within how many days?
3 days
58
T or F: It is fine to mix incompatible waste
False
59
T or F: It's okay to use the same funnel for containers with incompatible waste materials
False
60
T or F: Separately collect aqueous wastes from organic solvent wastes.
True
61
Glass is not used as a waste container if there is a presence of which danger?
Danger of freezing (can expand and break the glass)
62
T or F: In disposal of empty containers, rinsing properly is advised if necessary
True
63
Category of Health Care Waste; Not in contact with infectious agents, hazardous chemicals, radioactive materials
Non-hazardous or general waste
64
Category of Health Care Waste; refer to any specimen from a patient
Infectious wastes
65
Category of Health Care Waste; discarded needles used to give shots or draw blood and discarded lancets
Sharps
66
Category of Health Care Waste; Tissue sections and body material derived from biopsies or surgical procedures
Pathological waste
67
Category of Health Care Waste; Recognizable body parts usually from amputation procedures
Anatomical waste
68
Category of Health Care Waste; hazardous and non-hazardous substances from the laboratory
Chemical waste
69
Hazardous chemicals that are health and environment hazards
Toxic
70
Hazardous chemicals that are strong acids and bases
Corrosive
71
pH range of corrosive chemicals
less than 2.0 and/or greater than 12.0
72
Hazardous chemicals with flash point below 600 C
Flammable
73
Hazardous chemicals that are explosive with water
Reactive
74
Category of Health Care Waste; refers to containers that may explode
PRessurized containers
75
Category of Health Care Waste; radioactive or cytotoxic materials such as excreta of patients who underwent radionuclide procedures
Radioactive waste
76
Color of bin/liner of infectious waste
Yellow
77
Color of bin/liner of pathological and anatomical waste
Yellow
78
Color of bin/liner of sharps and pressurized containers
Red
79
Color of bin/liner of chemical waste
Yellow (with black band)
80
Color of bin/liner of pharmaceutical waste
Yellow (with black band)
81
Color of bin/liner of radioactive waste
Orange
82
Color of bin/liner of general waste (nonbiodegradable)
Black
83
Color of bin/liner of general waste (biodegradable)
Green
84
Refers to the process of changing the biological and chemical characteristics of waste to minimize its potential to cause harm
Treatment
85
Processes that refer to relying on heat to destroy pathogens
Thermal processes
86
Refers to thermal decomposition of health care wastes with absence of molecular oxygen
Pyrolysis
87
Where are residues of pyrolysis disposed?
Landfill
88
Refers to the use of steam sterilization with indicators
Autoclave
89
Heat and pressure of autoclave, and for how long
121 deg C, 15 psi for 15 - 30 mins
90
Refers to microorganisms being destroyed by heat which coagulates and denatures enzymes and proteins
Microwave
91
Temperature requirement for microwave and for how long
100 deg C for 30 mins
92
Solutions used in Chemical Disinfection treatment
Aldehydes, chlorine compounds, phenolic compounds such as Lysol
93
Refers to using enzyme mixture to decontaminate health care waste
Biological process
94
Kind of waste treatment that refers to breaking down the DNA molecules of the microorganism
Radiation Technology
95
Refers to filling up of containers with waste, adding immobilizing material, then sealing the container
Encapsulation
96
Which type of waste is commonly treated by encapsulation and inertization?
Pharmaceutical waste
97
Refers to a variant of encapsulation; pharmaceuticals ground then mix with water, cement and lime to form a homogenous paste
Inertization
98
Refers to an engineered site designed to keep waste isolated from the environment
Sanitary Landfill
99
Refers to where treated infectious wastes, sharps, pathological and anatomical wastes are disposed; health care facilities in remote areas
Safe burial
100
Refers to where sharps and syringes can be disposed
Septic or concrete vaults
101
Where are infectious wastes disposed?
Landfill
102
How is pathological waste disposed?
By burying
103
How is chemical waste disposed?
By recycling and draining down the sink (wtf)
104
How are radioactive wastes treated?
Waiting to decay
105
How are radioactive wastes disposed?
By the supplier
106
Where are aerosol and pressurized containers disposed?
By landfill or disposal by supplier
107
Where are sharps wastes disposed?
Landfill and by burying
108
Where are aerosol and pressurized containers disposed of?
By landfill or disposal by the supplier
109
How are expired pharmaceutical wastes disposed of?
By the supplier
110
You should wait how long to dispose serum/CSF/body fluid specimen?
48 hours
111
You should wait how long to dispose urine specimens?
24 hours
112
You should wait how long to dispose blood/fluid smears?
7 days
113
You should wait how long to dispose microbiology-stained slides?
7 days
114
You should wait how long to dispose surgical pathology slides?
10 years