(F) Lesson 5: Laboratory Assistant/Laboratory Waste Management Plan Flashcards
(114 cards)
T or F: Laboratory assistant have job assignments that vary from one institution to another
True
A laboratory assistant may be assigned into which two roles?
A. Receptionist in the reception area
B. Laboratory aide in testing areas
T or F: Laboratory glasswares should not only be clean physically but also chemically.
True
What is the common two-way rinse of washing laboratory glassware?
With tap and distilled/deionized water
How do you commonly dry laboratory glassware?
Air dry or with drying oven
In removing grease, you need to boil the glassware in which weak solutions?
Sodium carbonate, acetone, or any fat solution
For greasy stopcock in laboratory equipment, in which solution do you soak it and for how long?
In a warm decahydronaphthalene for 2 hours
In removing grease, what do you use to rinse it and for how long?
With acetone or fuming sulfuric acid for 30 mins
In rinsing, how many times do you fill with water, shake, and empty each test tube, graduated cylinder, and/or flask?
6 times
These are machines/a type of technology that can be used for cleansing pipets
Pipet washers
T or F: In washing pipets, place pipets with tips up in a cylinder or tall jar of water.
False (tips down)
If the pipet is dirty, where do you soak it?
In a jar of chromic acid cleaning solution
How long do you soak in distilled water the pipet after rinsing with tap water?
At least 1 hour
In washing, the culture tubes are autoclaved for how long?
30 mins
In washing, the culture tubes are autoclaved in which temperature and pressure setting?
121 deg C and 15 psi
What are used to sterilize Petri dishes and culture bottles?
Autoclave or a dry-air sterilizer
In sterilizing serology tubes, what do you add in the bucket before boiling?
1% - 2% soap or detergent
In sterilizing serology tubes, how long do you boil them?
30 mins
What is the drying temperature for serology tubes and culture media?
less than 140 deg C
Where do you hang serology tubes to dry?
Wooden pegs
T or F: Acidic liquids should not be stored in flasks because the stoppers or stopcocks may stick
False (alkaline)
Where do you document the cleaning and maintenance activities in the laboratory?
Laboratory logbook
T or F: The laboratory exit floor plan should have three clear passageways.
False (two)
T or F: When designing the location of safety showers, eyewashes, fire extinguishers, etc, aesthetic value should be considered
False (must be strategically placed)
